POLR2C Protein (AA 2-275) (His tag)

N° du produit ABIN3087390

Détail du produit

Antigène: POLR2C (Polymerase (RNA) II (DNA Directed) Polypeptide C, 33kDa (POLR2C))

Type de proteíne: Recombinant

Attributs du protein: AA 2-275

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette POLR2C protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: PYANQPTVRI TELTDENVKF IIENTDLAVA NSIRRVFIAE VPIIAIDWVQ IDANSSVLHD EFIAHRLGLI PLISDDIVDK LQYSRDCTCE EFCPECSVEF TLDVRCNEDQ TRHVTSRDLI SNSPRVIPVT SRNRDNDPND YVEQDDILIV KLRKGQELRL RAYAKKGFGK EHAKWNPTAG VAFEYDPDNA LRHTVYPKPE EWPKSEYSEL DEDESQAPYD PNGKPERFYY NVESCGSLRP ETIVLSALSG LKKKLSDLQT QLSHEIQSDV LTIN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human POLR2C Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: POLR2C (Polymerase (RNA) II (DNA Directed) Polypeptide C, 33kDa (POLR2C))

Autre désignation: POLR2C (POLR2C Produits)

Synonymes: RPB3 Protein, RPB31 Protein, hRPB33 Protein, hsRPB3 Protein, 33kDa Protein, Rpo2-3 Protein, mRBP31 Protein, zgc:56285 Protein, wu:fk48d01 Protein, MGC76329 Protein, MGC81245 Protein, POLR2C Protein, polr2c Protein, rpb3 Protein, RNA polymerase II subunit C Protein, polymerase (RNA) II (DNA directed) polypeptide C Protein, polymerase (RNA) II (DNA directed) polypeptide C, 33kDa Protein, polymerase (RNA) II (DNA directed) polypeptide C, 33kDa S homeolog Protein, POLR2C Protein, Polr2c Protein, polr2c Protein, polr2c.S Protein

Sujet: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity). {ECO:0000250, ECO:0000269|PubMed:9852112}.

Poids moléculaire: 32.3 kDa Including tag.

UniProt: P19387

Pathways: Regulatory RNA Pathways