GOT1 Protein (AA 2-413) (His tag)

N° du produit ABIN3088321

Détail du produit

Antigène: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-413

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette GOT1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: APPSVFAEVP QAQPVLVFKL TADFREDPDP RKVNLGVGAY RTDDCHPWVL PVVKKVEQKI ANDNSLNHEY LPILGLAEFR SCASRLALGD DSPALKEKRV GGVQSLGGTG ALRIGADFLA RWYNGTNNKN TPVYVSSPTW ENHNAVFSAA GFKDIRSYRY WDAEKRGLDL QGFLNDLENA PEFSIVVLHA CAHNPTGIDP TPEQWKQIAS VMKHRFLFPF FDSAYQGFAS GNLERDAWAI RYFVSEGFEF FCAQSFSKNF GLYNERVGNL TVVGKEPESI LQVLSQMEKI VRITWSNPPA QGARIVASTL SNPELFEEWT GNVKTMADRI LTMRSELRAR LEALKTPGTW NHITDQIGMF SFTGLNPKQV EYLVNEKHIY LLPSGRINVS GLTTKNLDYV ATSIHEAVTK IQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GOT1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Autre désignation: GOT1 (GOT1 Produits)

Synonymes: ASTQTL1 Protein, GIG18 Protein, cAspAT Protein, cCAT Protein, AST Protein, ISSD Protein, NSD Protein, SD Protein, SIALIN Protein, SIASD Protein, SLD Protein, xr406 Protein, CASPAT Protein, ASPARTATE AMINOTRANSFERASE Protein, ATAAT Protein, MATERNAL EFFECT EMBRYO ARREST 17 Protein, MEE17 Protein, T26C19.9 Protein, T26C19_9 Protein, aspartate aminotransferase Protein, aatA Protein, 83.t00021 Protein, AI789014 Protein, Got-1 Protein, Aspat Protein, Gaspat Protein, AL022787 Protein, FABP-pm Protein, Got-2 Protein, mAspAT Protein, ASPATA Protein, glutamic-oxaloacetic transaminase 1 Protein, solute carrier family 17 member 5 Protein, glutamic-oxaloacetic transaminase 1 homeolog Protein, aspartate aminotransferase Protein, glutamic-oxaloacetic transaminase 1, soluble Protein, glutamic-oxaloacetic transaminase 2 Protein, glutamatic-oxaloacetic transaminase 2, mitochondrial Protein, GOT1 Protein, SLC17A5 Protein, got1.S Protein, AAT Protein, aspC Protein, EHI_006080 Protein, AST2 Protein, ast2 Protein, got1 Protein, Got1 Protein, GOT2 Protein, Got2 Protein

Sujet: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. {ECO:0000269|PubMed:16039064}.

Poids moléculaire: 47.1 kDa Including tag.

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process