ARHGEF18 Protein (AA 1-1173) (His tag)

N° du produit ABIN3089154

Détail du produit

Antigène: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1173

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ARHGEF18 protéine est marqué à la His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Séquence: MVTVGTNILP SRPAASANTA REDAALFSRR IPPRHKNGAA QPGAAPGPGA PGANMGNAHS KSGDRHSALP GRPELSFYGS FPRKWSENVF LDNELLTSKI LSVLRPQSER GFRAGDLRYP THFLSTNSVL ASVTASLKEH PRGTLLSDGS PALSRNVGMT VSQKGGPQPT PSPAGPGTQL GPITGEMDEA DSAFLKFKQT ADDSLSLTSP NTESIFVEDP YTASLRSEIE SDGHEFEAES WSLAVDAAYA KKQKREVVKR QDVLYELMQT EVHHVRTLKI MLKVYSRALQ EELQFSSKAI GRLFPCADDL LETHSHFLAR LKERRQESLE EGSDRNYVIQ KIGDLLVQQF SGENGERMKE KYGVFCSGHN EAVSHYKLLL QQNKKFQNLI KKIGNFSIVR RLGVQECILL VTQRITKYPV LVERIIQNTE AGTEDYEDLT QALNLIKDII SQVDAKVSEC EKGQRLREIA GKMDLKSSSK LKNGLTFRKE DMLQRQLHLE GMLCWKTTSG RLKDILAILL TDVLLLLQEK DQKYVFASVD SKPPVISLQK LIVREVANEE KAMFLISASL QGPEMYEIYT SSKEDRNAWM AHIQRAVESC PDEEEGPFSL PEEERKVVEA RATRLRDFQE RLSMKDQLIA QSLLEKQQIY LEMAEMGGLE DLPQPRGLFR GGDPSETLQG ELILKSAMSE IEGIQSLICR QLGSANGQAE DGGSSTGPPR RAETFAGYDC TNSPTKNGSF KKKVSSTDPR PRDWRGPPNS PDLKLSDSDI PGSSEESPQV VEAPGTESDP RLPTVLESEL VQRIQTLSQL LLNLQAVIAH QDSYVETQRA AIQEREKQFR LQSTRGNLLL EQERQRNFEK QREERAALEK LQSQLRHEQQ RWERERQWQH QELERAGARL QEREGEARQL RERLEQERAE LERQRQAYQH DLERLREAQR AVERERERLE LLRRLKKQNT APGALPPDTL AEAQPPSHPP SFNGEGLEGP RVSMLPSGVG PEYAERPEVA RRDSAPTENR LAKSDVPIQL LSATNQFQRQ AAVQQQIPTK LAASTKGGKD KGGKSRGSQR WESSASFDLK QQLLLNKLMG KDESTSRNRR SLSPILPGRH SPAPPPDPGF PAPSPPPADS PSEGFSLKAG GTALLPGPPA PSPLPATPLS AKEDASKEDV IFF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ARHGEF18 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))

Autre désignation: ARHGEF18 (ARHGEF18 Produits)

Synonymes: arhgef18 Protein, wu:fc21e04 Protein, wu:fj05f11 Protein, zgc:85686 Protein, P114-RhoGEF Protein, AI467246 Protein, D030053O22Rik Protein, rho/rac guanine nucleotide exchange factor (GEF) 18a Protein, Rho/Rac guanine nucleotide exchange factor (GEF) 18 Protein, Rho/Rac guanine nucleotide exchange factor 18 Protein, rho guanine nucleotide exchange factor 18 Protein, rho/rac guanine nucleotide exchange factor (GEF) 18 Protein, arhgef18a Protein, ARHGEF18 Protein, LOC100027283 Protein, Arhgef18 Protein

Sujet: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). {ECO:0000269|PubMed:11085924, ECO:0000269|PubMed:14512443, ECO:0000269|PubMed:15558029, ECO:0000269|PubMed:22006950}.

Poids moléculaire: 131.7 kDa Including tag.

UniProt: Q6ZSZ5

Pathways: Neurotrophin Signaling Pathway