Complement Factor H Protein (CFH) (AA 19-1231) (His tag)

N° du produit ABIN3091041

Détail du produit

Antigène: Complement Factor H (CFH)

Type de proteíne: Recombinant

Attributs du protein: AA 19-1231

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Complement Factor H protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: EDCNELPPRR NTEILTGSWS DQTYPEGTQA IYKCRPGYRS LGNVIMVCRK GEWVALNPLR KCQKRPCGHP GDTPFGTFTL TGGNVFEYGV KAVYTCNEGY QLLGEINYRE CDTDGWTNDI PICEVVKCLP VTAPENGKIV SSAMEPDREY HFGQAVRFVC NSGYKIEGDE EMHCSDDGFW SKEKPKCVEI SCKSPDVING SPISQKIIYK ENERFQYKCN MGYEYSERGD AVCTESGWRP LPSCEEKSCD NPYIPNGDYS PLRIKHRTGD EITYQCRNGF YPATRGNTAK CTSTGWIPAP RCTLKPCDYP DIKHGGLYHE NMRRPYFPVA VGKYYSYYCD EHFETPSGSY WDHIHCTQDG WSPAVPCLRK CYFPYLENGY NQNYGRKFVQ GKSIDVACHP GYALPKAQTT VTCMENGWSP TPRCIRVKTC SKSSIDIENG FISESQYTYA LKEKAKYQCK LGYVTADGET SGSITCGKDG WSAQPTCIKS CDIPVFMNAR TKNDFTWFKL NDTLDYECHD GYESNTGSTT GSIVCGYNGW SDLPICYERE CELPKIDVHL VPDRKKDQYK VGEVLKFSCK PGFTIVGPNS VQCYHFGLSP DLPICKEQVQ SCGPPPELLN GNVKEKTKEE YGHSEVVEYY CNPRFLMKGP NKIQCVDGEW TTLPVCIVEE STCGDIPELE HGWAQLSSPP YYYGDSVEFN CSESFTMIGH RSITCIHGVW TQLPQCVAID KLKKCKSSNL IILEEHLKNK KEFDHNSNIR YRCRGKEGWI HTVCINGRWD PEVNCSMAQI QLCPPPPQIP NSHNMTTTLN YRDGEKVSVL CQENYLIQEG EEITCKDGRW QSIPLCVEKI PCSQPPQIEH GTINSSRSSQ ESYAHGTKLS YTCEGGFRIS EENETTCYMG KWSSPPQCEG LPCKSPPEIS HGVVAHMSDS YQYGEEVTYK CFEGFGIDGP AIAKCLGEKW SHPPSCIKTD CLSLPSFENA IPMGEKKDVY KAGEQVTYTC ATYYKMDGAS NVTCINSRWT GRPTCRDTSC VNPPTVQNAY IVSRQMSKYP SGERVRYQCR SPYEMFGDEE VMCLNGNWTE PPQCKDSTGK CGPPPPIDNG DITSFPLSVY APASSVEYQC QNLYQLEGNK RITCRNGQWS EPPKCLHPCV ISREIMENYN IALRWTAKQK LYSRTGESVE FVCKRGYRLS SRSHTLRTTC WDGKLEYPTC AKR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CFH Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Complement Factor H (CFH)

Autre désignation: CFH (CFH Produits)

Synonymes: AHUS1 Protein, AMBP1 Protein, ARMD4 Protein, ARMS1 Protein, CFHL3 Protein, FH Protein, FHL1 Protein, HF Protein, HF1 Protein, HF2 Protein, HUS Protein, Mud-1 Protein, NOM Protein, Sas-1 Protein, Sas1 Protein, Fh Protein, CFH Protein, im:7147412 Protein, si:ch211-207o17.2 Protein, fH Protein, armd4 Protein, arms1 Protein, cfh Protein, cfhl3 Protein, fhl1 Protein, hf1 Protein, hf2 Protein, hus Protein, complement factor H Protein, complement component factor h Protein, complement factor H-related protein 5 Protein, complement factor H L homeolog Protein, CFH Protein, Cfh Protein, cfh Protein, LOC100399888 Protein, LOC100733902 Protein, cfh.L Protein

Sujet: Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.

Poids moléculaire: 138.0 kDa Including tag.

UniProt: P08603

Pathways: Système du Complément, Cellular Response to Molecule of Bacterial Origin