HNRNPUL1 Protein (AA 1-856) (Strep Tag)

N° du produit ABIN3092955

Détail du produit

Antigène: HNRNPUL1 (Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-856

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette HNRNPUL1 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MDVRRLKVNE LREELQRRGL DTRGLKAELA ERLQAALEAE EPDDERELDA DDEPGRPGHI NEEVETEGGS ELEGTAQPPP PGLQPHAEPG GYSGPDGHYA MDNITRQNQF YDTQVIKQEN ESGYERRPLE MEQQQAYRPE MKTEMKQGAP TSFLPPEASQ LKPDRQQFQS RKRPYEENRG RGYFEHREDR RGRSPQPPAE EDEDDFDDTL VAIDTYNCDL HFKVARDRSS GYPLTIEGFA YLWSGARASY GVRRGRVCFE MKINEEISVK HLPSTEPDPH VVRIGWSLDS CSTQLGEEPF SYGYGGTGKK STNSRFENYG DKFAENDVIG CFADFECGND VELSFTKNGK WMGIAFRIQK EALGGQALYP HVLVKNCAVE FNFGQRAEPY CSVLPGFTFI QHLPLSERIR GTVGPKSKAE CEILMMVGLP AAGKTTWAIK HAASNPSKKY NILGTNAIMD KMRVMGLRRQ RNYAGRWDVL IQQATQCLNR LIQIAARKKR NYILDQTNVY GSAQRRKMRP FEGFQRKAIV ICPTDEDLKD RTIKRTDEEG KDVPDHAVLE MKANFTLPDV GDFLDEVLFI ELQREEADKL VRQYNEEGRK AGPPPEKRFD NRGGGGFRGR GGGGGFQRYE NRGPPGGNRG GFQNRGGGSG GGGNYRGGFN RSGGGGYSQN RWGNNNRDNN NSNNRGSYNR APQQQPPPQQ PPPPQPPPQQ PPPPPSYSPA RNPPGASTYN KNSNIPGSSA NTSTPTVSSY SPPQPSYSQP PYNQGGYSQG YTAPPPPPPP PPAYNYGSYG GYNPAPYTPP PPPTAQTYPQ PSYNQYQQYA QQWNQYYQNQ GQWPPYYGNY DYGSYSGNTQ GGTSTQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: HNRNPUL1 (Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1))

Autre désignation: HNRNPUL1 (HNRNPUL1 Produits)

Synonymes: HNRPUL1 Protein, E1B-AP5 Protein, E1BAP5 Protein, E130317O14Rik Protein, Hnrnpul Protein, Hnrpul1 Protein, hnrpul1 Protein, wu:fb53d10 Protein, wu:fk45c03 Protein, zgc:85971 Protein, heterogeneous nuclear ribonucleoprotein U like 1 Protein, coiled-coil domain containing 97 Protein, heterogeneous nuclear ribonucleoprotein U-like 1 Protein, HNRNPUL1 Protein, CCDC97 Protein, Desac_1308 Protein, Hnrnpul1 Protein, hnrnpul1 Protein

Sujet: Heterogeneous nuclear ribonucleoprotein U-like protein 1 (Adenovirus early region 1B-associated protein 5) (E1B-55 kDa-associated protein 5) (E1B-AP5),FUNCTION: Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Also plays a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro. {ECO:0000269|PubMed:12489984, ECO:0000269|PubMed:9733834}.

Poids moléculaire: 95.7 kDa

UniProt: Q9BUJ2