HTATSF1 Protein (AA 2-755) (His tag)

N° du produit ABIN3093011

Détail du produit

Antigène: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-755

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette HTATSF1 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: SGTNLDGNDE FDEQLRMQEL YGDGKDGDTQ TDAGGEPDSL GQQPTDTPYE WDLDKKAWFP KITEDFIATY QANYGFSNDG ASSSTANVED VHARTAEEPP QEKAPEPTDA RKKGEKRKAE SGWFHVEEDR NTNVYVSGLP PDITVDEFIQ LMSKFGIIMR DPQTEEFKVK LYKDNQGNLK GDGLCCYLKR ESVELALKLL DEDEIRGYKL HVEVAKFQLK GEYDASKKKK KCKDYKKKLS MQQKQLDWRP ERRAGPSRMR HERVVIIKNM FHPMDFEDDP LVLNEIREDL RVECSKFGQI RKLLLFDRHP DGVASVSFRD PEEADYCIQT LDGRWFGGRQ ITAQAWDGTT DYQVEETSRE REERLRGWEA FLNAPEANRG LRRSDSVSAS ERAGPSRARH FSEHPSTSKM NAQETATGMA FEEPIDEKKF EKTEDGGEFE EGASENNAKE SSPEKEAEEG CPEKESEEGC PKRGFEGSCS QKESEEGNPV RGSEEDSPKK ESKKKTLKND CEENGLAKES EDDLNKESEE EVGPTKESEE DDSEKESDED CSEKQSEDGS EREFEENGLE KDLDEEGSEK ELHENVLDKE LEENDSENSE FEDDGSEKVL DEEGSEREFD EDSDEKEEEE DTYEKVFDDE SDEKEDEEYA DEKGLEAADK KAEEGDADEK LFEESDDKED EDADGKEVED ADEKLFEDDD SNEKLFDEEE DSSEKLFDDS DERGTLGGFG SVEEGPLSTG SSFILSSDDD DDDI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HTATSF1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Autre désignation: HTATSF1 (HTATSF1 Produits)

Synonymes: zgc:66252 Protein, MGC98898 Protein, HTATSF1 Protein, DKFZp468B086 Protein, 1600023H17Rik Protein, 2600017A12Rik Protein, 2700077B20Rik Protein, TAT-SF1 Protein, TATSF1 Protein, dJ196E23.2 Protein, RGD1563377 Protein, HIV-1 Tat specific factor 1 Protein, HIV-1 Tat specific factor 1 S homeolog Protein, HIV Tat-specific factor 1 homolog Protein, HIV TAT specific factor 1 Protein, htatsf1 Protein, htatsf1.S Protein, HTATSF1 Protein, LOC778641 Protein, Htatsf1 Protein

Sujet: Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus. {ECO:0000269|PubMed:10393184, ECO:0000269|PubMed:10454543, ECO:0000269|PubMed:10913173, ECO:0000269|PubMed:11420046, ECO:0000269|PubMed:11780068, ECO:0000269|PubMed:15905670, ECO:0000269|PubMed:8849451, ECO:0000269|PubMed:9765201}.

Poids moléculaire: 86.7 kDa Including tag.

UniProt: O43719