Importin 9 Protein (IPO9) (AA 2-1041) (His tag)

N° du produit ABIN3093082

Détail du produit

Antigène: Importin 9 (IPO9)

Type de proteíne: Recombinant

Attributs du protein: AA 2-1041

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Importin 9 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: AAAAAAGAAS GLPGPVAQGL KEALVDTLTG ILSPVQEVRA AAEEQIKVLE VTEEFGVHLA ELTVDPQGAL AIRQLASVIL KQYVETHWCA QSEKFRPPET TERAKIVIRE LLPNGLRESI SKVRSSVAYA VSAIAHWDWP EAWPQLFNLL MEMLVSGDLN AVHGAMRVLT EFTREVTDTQ MPLVAPVILP EMYKIFTMAE VYGIRTRSRA VEIFTTCAHM ICNMEELEKG AAKVLIFPVV QQFTEAFVQA LQIPDGPTSD SGFKMEVLKA VTALVKNFPK HMVSSMQQIL PIVWNTLTES AAFYVRTEVN YTEEVEDPVD SDGEVLGFEN LVFSIFEFVH ALLENSKFKS TVKKALPELI YYIILYMQIT EEQIKVWTAN PQQFVEDEDD DTFSYTVRIA AQDLLLAVAT DFQNESAAAL AAAATRHLQE AEQTKNSGTE HWWKIHEACM LALGSVKAII TDSVKNGRIH FDMHGFLTNV ILADLNLSVS PFLLGRALWA ASRFTVAMSP ELIQQFLQAT VSGLHETQPP SVRISAVRAI WGYCDQLKVS ESTHVLQPFL PSILDGLIHL AAQFSSEVLN LVMETLCIVC TVDPEFTASM ESKICPFTIA IFLKYSNDPV VASLAQDIFK ELSQIEACQG PMQMRLIPTL VSIMQAPADK IPAGLCATAI DILTTVVRNT KPPLSQLLIC QAFPAVAQCT LHTDDNATMQ NGGECLRAYV SVTLEQVAQW HDEQGHNGLW YVMQVVSQLL DPRTSEFTAA FVGRLVSTLI SKAGRELGEN LDQILRAILS KMQQAETLSV MQSLIMVFAH LVHTQLEPLL EFLCSLPGPT GKPALEFVMA EWTSRQHLFY GQYEGKVSSV ALCKLLQHGI NADDKRLQDI RVKGEEIYSM DEGIRTRSKS AKNPERWTNI PLLVKILKLI INELSNVMEA NAARQATPAE WSQDDSNDMW EDQEEEEEEE EDGLAGQLLS DILATSKYEE DYYEDDEEDD PDALKDPLYQ IDLQAYLTDF LCQFAQQPCY IMFSGHLNDN ERRVLQTIGI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human IPO9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Importin 9 (IPO9)

Autre désignation: IPO9 (IPO9 Produits)

Synonymes: Imp9 Protein, 0710008K06Rik Protein, AI845704 Protein, C78347 Protein, Ranbp9 Protein, mKIAA1192 Protein, zgc:66429 Protein, wu:fc23d12 Protein, MGC81741 Protein, IPO9 Protein, DDBDRAFT_0205722 Protein, DDBDRAFT_0229883 Protein, DDB_0205722 Protein, DDB_0229883 Protein, RGD1561940 Protein, RanBPM Protein, RanBP9 Protein, bpm-l Protein, bpm90 Protein, ranbp9 Protein, ranbp9-a Protein, ranbp9-b Protein, ranbp9b Protein, cb457 Protein, zgc:158650 Protein, importin 9 Protein, importin 9 L homeolog Protein, RAN binding protein 9 Protein, RAN binding protein 9 L homeolog Protein, IPO9 Protein, Ipo9 Protein, ipo9 Protein, ipo9.L Protein, cnrP Protein, Ranbp9 Protein, ranbp9.L Protein, RANBP9 Protein, ranbp9 Protein

Sujet: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6. {ECO:0000250, ECO:0000269|PubMed:11823430}.

Poids moléculaire: 116.8 kDa Including tag.

UniProt: Q96P70

Pathways: Protein targeting to Nucleus