MCF2L Protein (AA 1-1137) (Strep Tag)

N° du produit ABIN3093789

Détail du produit

Antigène: MCF2L (MCF.2 Cell Line Derived Transforming Sequence-Like (MCF2L))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1137

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MCF2L protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MFDCWRFILC KRPGSNSYSS PQRPNEAKKE ETDHQIDVSD VIRLVQDTPE ATAMATDEIM HQDIVPLCAA DIQDQLKKRF AYLSGGRGQD GSPVITFPDY PAFSEIPDKE FQNVMTYLTS IPSLQDAGIG FILVIDRRRD KWTSVKASVL RIAASFPANL QLVLVLRPTG FFQRTLSDIA FKFNRDDFKM KVPVIMLSSV PDLHGYIDKS QLTEDLGGTL DYCHSRWLCQ RTAIESFALM VKQTAQMLQS FGTELAETEL PNDVQSTSSV LCAHTEKKDK AKEDLRLALK EGHSVLESLR ELQAEGSEPS VNQDQLDNQA TVQRLLAQLN ETEAAFDEFW AKHQQKLEQC LQLRHFEQGF REVKAILDAA SQKIATFTDI GNSLAHVEHL LRDLASFEEK SGVAVERARA LSLDGEQLIG NKHYAVDSIR PKCQELRHLC DQFSAEIARR RGLLSKSLEL HRRLETSMKW CDEGIYLLAS QPVDKCQSQD GAEAALQEIE KFLETGAENK IQELNAIYKE YESILNQDLM EHVRKVFQKQ ASMEEVFHRR QASLKKLAAR QTRPVQPVAP RPEALAKSPC PSPGIRRGSE NSSSEGGALR RGPYRRAKSE MSESRQGRGS AGEEEESLAI LRRHVMSELL DTERAYVEEL LCVLEGYAAE MDNPLMAHLL STGLHNKKDV LFGNMEEIYH FHNRIFLREL ENYTDCPELV GRCFLERMED FQIYEKYCQN KPRSESLWRQ CSDCPFFQEC QRKLDHKLSL DSYLLKPVQR ITKYQLLLKE MLKYSRNCEG AEDLQEALSS ILGILKAVND SMHLIAITGY DGNLGDLGKL LMQGSFSVWT DHKRGHTKVK ELARFKPMQR HLFLHEKAVL FCKKREENGE GYEKAPSYSY KQSLNMAAVG ITENVKGDAK KFEIWYNARE EVYIVQAPTP EIKAAWVNEI RKVLTSQLQA CREASQHRAL EQSQSLPLPA PTSTSPSRGN SRNIKKLEER KTDPLSLEGY VSSAPLTKPP EKGKGWSKTS HSLEAPEDDG GWSSAEEQIN SSDAEEDGGL GPKKLVPGKY TVVADHEKGG PDALRVRSGD VVELVQEGDE GLWYVRDPTT GKEGWVPASS LSVRLGPSGS AQCLSSSGKA HVPRAHP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MCF2L (MCF.2 Cell Line Derived Transforming Sequence-Like (MCF2L))

Autre désignation: MCF2L (MCF2L Produits)

Synonymes: ARHGEF14 Protein, DBS Protein, OST Protein, Ost Protein, mcf 2l Protein, MCF2L Protein, NV11503 Protein, dbs Protein, ost Protein, arhgef14 Protein, C130040G20Rik Protein, Dbs Protein, mKIAA0362 Protein, MCF.2 cell line derived transforming sequence like Protein, MCF.2 cell line derived transforming sequence-like Protein, guanine nucleotide exchange factor DBS Protein, mcf.2 transforming sequence-like Protein, MCF2L Protein, Mcf2l Protein, LOC100122691 Protein, mcf2l.2 Protein, mcf2l Protein

Sujet: Guanine nucleotide exchange factor DBS (DBL's big sister) (MCF2-transforming sequence-like protein),FUNCTION: Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42 (PubMed:15157669). {ECO:0000250|UniProtKB:Q63406, ECO:0000269|PubMed:15157669}., FUNCTION: [Isoform 3]: Does not catalyze guanine nucleotide exchange on CDC42 (PubMed:15157669). {ECO:0000269|PubMed:15157669}.

Poids moléculaire: 128.1 kDa

UniProt: O15068

Pathways: Neurotrophin Signaling Pathway, Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis