MPRIP Protein (AA 2-1025) (His tag)

N° du produit ABIN3093913

Détail du produit

Antigène: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1025

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette MPRIP protéine est marqué à la His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Séquence: SAAKENPCRK FQANIFNKSK CQNCFKPRES HLLNDEDLTQ AKPIYGGWLL LAPDGTDFDN PVHRSRKWQR RFFILYEHGL LRYALDEMPT TLPQGTINMN QCTDVVDGEG RTGQKFSLCI LTPEKEHFIR AETKEIVSGW LEMLMVYPRT NKQNQKKKRK VEPPTPQEPG PAKVAVTSSS SSSSSSSSIP SAEKVPTTKS TLWQEEMRTK DQPDGSSLSP AQSPSQSQPP AASSLREPGL ESKEEESAMS SDRMDCGRKV RVESGYFSLE KTKQDLKAEE QQLPPPLSPP SPSTPNHRRS QVIEKFEALD IEKAEHMETN AVGPSPSSDT RQGRSEKRAF PRKRDFTNEA PPAPLPDASA SPLSPHRRAK SLDRRSTEPS VTPDLLNFKK GWLTKQYEDG QWKKHWFVLA DQSLRYYRDS VAEEAADLDG EIDLSACYDV TEYPVQRNYG FQIHTKEGEF TLSAMTSGIR RNWIQTIMKH VHPTTAPDVT SSLPEEKNKS SCSFETCPRP TEKQEAELGE PDPEQKRSRA RERRREGRSK TFDWAEFRPI QQALAQERVG GVGPADTHEP LRPEAEPGEL ERERARRREE RRKRFGMLDA TDGPGTEDAA LRMEVDRSPG LPMSDLKTHN VHVEIEQRWH QVETTPLREE KQVPIAPVHL SSEDGGDRLS THELTSLLEK ELEQSQKEAS DLLEQNRLLQ DQLRVALGRE QSAREGYVLQ ATCERGFAAM EETHQKKIED LQRQHQRELE KLREEKDRLL AEETAATISA IEAMKNAHRE EMERELEKSQ RSQISSVNSD VEALRRQYLE ELQSVQRELE VLSEQYSQKC LENAHLAQAL EAERQALRQC QRENQELNAH NQELNNRLAA EITRLRTLLT GDGGGEATGS PLAQGKDAYE LEVLLRVKES EIQYLKQEIS SLKDELQTAL RDKKYASDKY KDIYTELSIA KAKADCDISR LKEQLKAATE ALGEKSPDSA TVSGYDIMKS KSNPDFLKKD RSCVTRQLRN IRSKSVIEQV SWDT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human MPRIP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Autre désignation: MPRIP (MPRIP Produits)

Synonymes: M-RIP Protein, MRIP Protein, RHOIP3 Protein, RIP3 Protein, p116Rip Protein, 9530046C02 Protein, AA536749 Protein, AI647711 Protein, C76423 Protein, Rhoip3 Protein, mKIAA0864 Protein, M-rip Protein, Mrip Protein, myosin phosphatase Rho interacting protein Protein, MPRIP Protein, Mprip Protein

Sujet: Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. {ECO:0000250|UniProtKB:P97434, ECO:0000269|PubMed:15545284, ECO:0000269|PubMed:16257966}.

Poids moléculaire: 117.4 kDa Including tag.

UniProt: Q6WCQ1