NCOA3 Protein (AA 2-1424) (His tag)

N° du produit ABIN3094095

Détail du produit

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1424

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette NCOA3 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: SGLGENLDPL ASDSRKRKLP CDTPGQGLTC SGEKRRREQE SKYIEELAEL ISANLSDIDN FNVKPDKCAI LKETVRQIRQ IKEQGKTISN DDDVQKADVS STGQGVIDKD SLGPLLLQAL DGFLFVVNRD GNIVFVSENV TQYLQYKQED LVNTSVYNIL HEEDRKDFLK NLPKSTVNGV SWTNETQRQK SHTFNCRMLM KTPHDILEDI NASPEMRQRY ETMQCFALSQ PRAMMEEGED LQSCMICVAR RITTGERTFP SNPESFITRH DLSGKVVNID TNSLRSSMRP GFEDIIRRCI QRFFSLNDGQ SWSQKRHYQE AYLNGHAETP VYRFSLADGT IVTAQTKSKL FRNPVTNDRH GFVSTHFLQR EQNGYRPNPN PVGQGIRPPM AGCNSSVGGM SMSPNQGLQM PSSRAYGLAD PSTTGQMSGA RYGGSSNIAS LTPGPGMQSP SSYQNNNYGL NMSSPPHGSP GLAPNQQNIM ISPRNRGSPK IASHQFSPVA GVHSPMASSG NTGNHSFSSS SLSALQAISE GVGTSLLSTL SSPGPKLDNS PNMNITQPSK VSNQDSKSPL GFYCDQNPVE SSMCQSNSRD HLSDKESKES SVEGAENQRG PLESKGHKKL LQLLTCSSDD RGHSSLTNSP LDSSCKESSV SVTSPSGVSS STSGGVSSTS NMHGSLLQEK HRILHKLLQN GNSPAEVAKI TAEATGKDTS SITSCGDGNV VKQEQLSPKK KENNALLRYL LDRDDPSDAL SKELQPQVEG VDNKMSQCTS STIPSSSQEK DPKIKTETSE EGSGDLDNLD AILGDLTSSD FYNNSISSNG SHLGTKQQVF QGTNSLGLKS SQSVQSIRPP YNRAVSLDSP VSVGSSPPVK NISAFPMLPK QPMLGGNPRM MDSQENYGSS MGGPNRNVTV TQTPSSGDWG LPNSKAGRME PMNSNSMGRP GGDYNTSLPR PALGGSIPTL PLRSNSIPGA RPVLQQQQQM LQMRPGEIPM GMGANPYGQA AASNQLGSWP DGMLSMEQVS HGTQNRPLLR NSLDDLVGPP SNLEGQSDER ALLDQLHTLL SNTDATGLEE IDRALGIPEL VNQGQALEPK QDAFQGQEAA VMMDQKAGLY GQTYPAQGPP MQGGFHLQGQ SPSFNSMMNQ MNQQGNFPLQ GMHPRANIMR PRTNTPKQLR MQLQQRLQGQ QFLNQSRQAL ELKMENPTAG GAAVMRPMMQ PQVSSQQGFL NAQMVAQRSR ELLSHHFRQQ RVAMMMQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQTQAFS PPPNVTASPS MDGLLAGPTM PQAPPQQFPY QPNYGMGQQP DPAFGRVSSP PNAMMSSRMG PSQNPMMQHP QAASIYQSSE MKGWPSGNLA RNSSFSQQQF AHQGNPAVYS MVHMNGSSGH MGQMNMNPMP MSGMPMGPDQ KYC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NCOA3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Autre désignation: NCOA3 (NCOA3 Produits)

Synonymes: NCOA3 Protein, ACTR Protein, AIB-1 Protein, AIB1 Protein, CAGH16 Protein, CTG26 Protein, KAT13B Protein, RAC3 Protein, SRC-3 Protein, SRC3 Protein, TNRC14 Protein, TNRC16 Protein, TRAM-1 Protein, bHLHe42 Protein, pCIP Protein, 2010305B15Rik Protein, AW321064 Protein, Actr Protein, Aib1 Protein, Rac3 Protein, Src3 Protein, Tram-1 Protein, Tram1 Protein, p/Cip Protein, pCip Protein, actr Protein, aib-1 Protein, aib1 Protein, cagh16 Protein, ctg26 Protein, kat13b Protein, pcip Protein, rac3 Protein, src-3 Protein, src3 Protein, tnrc14 Protein, tnrc16 Protein, tram-1 Protein, xSRC-3 Protein, nuclear receptor coactivator 3 Protein, nuclear receptor coactivator 3 S homeolog Protein, NCOA3 Protein, Ncoa3 Protein, ncoa3.S Protein

Sujet: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

Poids moléculaire: 156.1 kDa Including tag.

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha