Neuralized Homolog 4 (Drosophila) (NEURL4) (AA 1-1562) protein (Strep Tag)

N° du produit ABIN3094140

Détail du produit

Antigène: Neuralized Homolog 4 (Drosophila) (NEURL4)

Type de proteíne: Recombinant

Attributs du protein: AA 1-1562

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MAAGSGGSGG SGGGPGPGPG GGGGPSGSGS GPGSNGGLGS GGELHPRTGR LVSLSACGRT ARRQQPGQEF NHGLVLSREP LRDGRVFTVR IDRKVNSWSG SIEIGVTALD PSVLDFPSSA TGLKGGSWVV SGCSVLRDGR SVLEEYGQDL DQLGEGDRVG VERTVAGELR LWVNGRDCGV AATGLPPRVW AVVDLYGKCT QITVLPPEPG FSPPTPIPTP PLEPLAPTED SALAEQGTSA DEAFMVSPAQ ARPETFPNSL ESHNDFANME LSEVVSNTIL SAYNGGLLNV NLSSPPAGEG LGSSGAATSP ILTSNDALLF HEKCGTLIKL SNNNKTAERR RPLDEFNNGV VMTNRPLRDN EMFEIRIDKL VDKWSGSIEI GVTTHNPNSL EYPATMTNLQ SGTIMMSGCG ILTNGKGTRR EYCEFSLDEL QEGDHIGLTR KSNSALHFFI NGIDQGVATP LTPPVVYGVV DLYGMAVKVT IVHNNNHSDR LRRNNAILRA LSPEGALRRA APAAQAEPER LLFHPNCGQK AAITHEGRTA LRPHATDDFN HGVVLSSRAL RDGEVFQVRI DKMVDKWAGS IEIGVTTHNP AYLQLPSTMT NLRSGTWMMT GNGVMHNGTT ILDEYGHNLD RLKAGDTVGV VRREDGTLHF FVNGMTQGPA AWNVPPGVYA VVDLYGQAAQ ATIVDDVEVA PVPEPLPEGN NQVSPSSPSS GAGGSDLRFH QLHGSNAVIT NGGRTALRHN CRSEFNDAIV ISNRALRDGE LFEIVIQKMV DRWSGSIEAG VTAIRPEDLE FPNTMTDIDY DTWMLSGTAI MQDGNTMRNN YGCDLDALGT GARIGMMRTA KGDLHYFING QDQGAACSGL PPGKEVYAVV DLYGQCVQVS ITNATGPMDN SLATSNTATE KSFPLHSPVA GVAHRFHSTC GKNVTLEEDG TRAVRAAGYA HGLVFSTKEL RAEEVFEVKV EELDEKWAGS LRLGLTTLAP GEMGPGAGGG GPGLPPSLPE LRTKTTWMVS SCEVRRDGQL QRMNYGRNLE RLGVGSRVGV RRGADDTMHI LVDGEDMGPA ATGIAKNVWA VLDLYGPVRG VSIVSSTRLE ESEGTQPPSP SSDTGSEGEE DDEGEEHGLG GQNEVGIIPT TLEFLENHGK NILLSNGNRT ATRVASYNQG IVVINQPLVP QLLVQVRIDF LNRQWTSSLV LGVITCAPER LNFPASACAL KRAAWLLRGR GVFHNGLKIC EKFGPNLDTC PEGTILGLRL DSSGGLHLHV NGVDQGVAVP DVPQPCHALV DLYGQCEQVT IVNPEPGAAS GKSAGTQGDM EKADMVDGIK ESVCWGPPPA ASPLKSCEYH ALCSRFQELL LLPEDYFMPP PKRSLCYCES CRKLRGDEAH RRRGEPPREY ALPFGWCRFN LRVNPRLEAG TLTKKWHMAY HGSNVAAVRR VLDRGELGAG TASILSCRPL KGEPGVGFEE PGENCAPPRE EQPPPVLLSP SLQYAGAETL ASKVQFRDPK SQRTHQAQVA FQVCVRPGSY TPGPPSAALG EPPDPHFSPA ELEWVTKEKG ATLLCALLVR VE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Neuralized Homolog 4 (Drosophila) (NEURL4)

Autre désignation: NEURL4 (NEURL4 Produits)

Synonymes: 0610025P10Rik Protein, BC023037 Protein, RP23-172M21.17 Protein, neuralized E3 ubiquitin protein ligase 4 Protein, NEURL4 Protein, Neurl4 Protein

Sujet: Neuralized-like protein 4,FUNCTION: Promotes CCP110 ubiquitination and proteasome-dependent degradation. By counteracting accumulation of CP110, maintains normal centriolar homeostasis and preventing formation of ectopic microtubular organizing centers. {ECO:0000269|PubMed:22261722, ECO:0000269|PubMed:22441691}.

Poids moléculaire: 166.9 kDa

UniProt: Q96JN8