PTPRZ1 Protein (AA 25-1636) (His tag)

N° du produit ABIN3094845

Détail du produit

Antigène: PTPRZ1 (Protein tyrosine Phosphatase, Receptor-Type, Z Polypeptide 1 (PTPRZ1))

Type de proteíne: Recombinant

Attributs du protein: AA 25-1636

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PTPRZ1 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: YYRQQRKLVE EIGWSYTGAL NQKNWGKKYP TCNSPKQSPI NIDEDLTQVN VNLKKLKFQG WDKTSLENTF IHNTGKTVEI NLTNDYRVSG GVSEMVFKAS KITFHWGKCN MSSDGSEHSL EGQKFPLEMQ IYCFDADRFS SFEEAVKGKG KLRALSILFE VGTEENLDFK AIIDGVESVS RFGKQAALDP FILLNLLPNS TDKYYIYNGS LTSPPCTDTV DWIVFKDTVS ISESQLAVFC EVLTMQQSGY VMLMDYLQNN FREQQYKFSR QVFSSYTGKE EIHEAVCSSE PENVQADPEN YTSLLVTWER PRVVYDTMIE KFAVLYQQLD GEDQTKHEFL TDGYQDLGAI LNNLLPNMSY VLQIVAICTN GLYGKYSDQL IVDMPTDNPE LDLFPELIGT EEIIKEEEEG KDIEEGAIVN PGRDSATNQI RKKEPQISTT THYNRIGTKY NEAKTNRSPT RGSEFSGKGD VPNTSLNSTS QPVTKLATEK DISLTSQTVT ELPPHTVEGT SASLNDGSKT VLRSPHMNLS GTAESLNTVS ITEYEEESLL TSFKLDTGAE DSSGSSPATS AIPFISENIS QGYIFSSENP ETITYDVLIP ESARNASEDS TSSGSEESLK DPSMEGNVWF PSSTDITAQP DVGSGRESFL QTNYTEIRVD ESEKTTKSFS AGPVMSQGPS VTDLEMPHYS TFAYFPTEVT PHAFTPSSRQ QDLVSTVNVV YSQTTQPVYN GETPLQPSYS SEVFPLVTPL LLDNQILNTT PAASSSDSAL HATPVFPSVD VSFESILSSY DGAPLLPFSS ASFSSELFRH LHTVSQILPQ VTSATESDKV PLHASLPVAG GDLLLEPSLA QYSDVLSTTH AASETLEFGS ESGVLYKTLM FSQVEPPSSD AMMHARSSGP EPSYALSDNE GSQHIFTVSY SSAIPVHDSV GVTYQGSLFS GPSHIPIPKS SLITPTASLL QPTHALSGDG EWSGASSDSE FLLPDTDGLT ALNISSPVSV AEFTYTTSVF GDDNKALSKS EIIYGNETEL QIPSFNEMVY PSESTVMPNM YDNVNKLNAS LQETSVSISS TKGMFPGSLA HTTTKVFDHE ISQVPENNFS VQPTHTVSQA SGDTSLKPVL SANSEPASSD PASSEMLSPS TQLLFYETSA SFSTEVLLQP SFQASDVDTL LKTVLPAVPS DPILVETPKV DKISSTMLHL IVSNSASSEN MLHSTSVPVF DVSPTSHMHS ASLQGLTISY ASEKYEPVLL KSESSHQVVP SLYSNDELFQ TANLEINQAH PPKGRHVFAT PVLSIDEPLN TLINKLIHSD EILTSTKSSV TGKVFAGIPT VASDTFVSTD HSVPIGNGHV AITAVSPHRD GSVTSTKLLF PSKATSELSH SAKSDAGLVG GGEDGDTDDD GDDDDDDRGS DGLSIHKCMS CSSYRESQEK VMNDSDTHEN SLMDQNNPIS YSLSENSEED NRVTSVSSDS QTGMDRSPGK SPSANGLSQK HNDGKEENDI QTGSALLPLS PESKAWAVLT SDEESGSGQG TSDSLNENET STDFSFADTN EKDADGILAA GDSEITPGFP QSPTSSVTSE NSEVFHVSEA EASNSSHESR IGLAEGLESE KK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PTPRZ1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PTPRZ1 (Protein tyrosine Phosphatase, Receptor-Type, Z Polypeptide 1 (PTPRZ1))

Autre désignation: PTPRZ1 (PTPRZ1 Produits)

Synonymes: HPTPZ Protein, HPTPzeta Protein, PTP-ZETA Protein, PTP18 Protein, PTPRZ Protein, PTPZ Protein, R-PTP-zeta-2 Protein, RPTPB Protein, RPTPbeta Protein, phosphacan Protein, Ptpz Protein, AI850339 Protein, DSD-1-PG Protein, PTPbeta Protein, PTPzeta Protein, Ptprz Protein, RPTPz Protein, Rptpbeta Protein, protein tyrosine phosphatase, receptor type Z1 Protein, protein tyrosine phosphatase, receptor type Z, polypeptide 1 Protein, PTPRZ1 Protein, Ptprz1 Protein

Sujet: Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades (By similarity). {ECO:0000250}.

Poids moléculaire: 175.7 kDa Including tag.

UniProt: P23471