RBBP7 Protein (AA 2-425) (His tag)

N° du produit ABIN3094900

Détail du produit

Antigène: RBBP7 (Retinoblastoma Binding Protein 7 (RBBP7))

Type de proteíne: Recombinant

Attributs du protein: AA 2-425

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette RBBP7 protéine est marqué à la His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Séquence: ASKEMFEDTV EERVINEEYK IWKKNTPFLY DLVMTHALQW PSLTVQWLPE VTKPEGKDYA LHWLVLGTHT SDEQNHLVVA RVHIPNDDAQ FDASHCDSDK GEFGGFGSVT GKIECEIKIN HEGEVNRARY MPQNPHIIAT KTPSSDVLVF DYTKHPAKPD PSGECNPDLR LRGHQKEGYG LSWNSNLSGH LLSASDDHTV CLWDINAGPK EGKIVDAKAI FTGHSAVVED VAWHLLHESL FGSVADDQKL MIWDTRSNTT SKPSHLVDAH TAEVNCLSFN PYSEFILATG SADKTVALWD LRNLKLKLHT FESHKDEIFQ VHWSPHNETI LASSGTDRRL NVWDLSKIGE EQSAEDAEDG PPELLFIHGG HTAKISDFSW NPNEPWVICS VSEDNIMQIW QMAENIYNDE ESDVTTSELE GQGS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RBBP7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RBBP7 (Retinoblastoma Binding Protein 7 (RBBP7))

Autre désignation: RBBP7 (RBBP7 Produits)

Synonymes: RbAp46 Protein, AA409861 Protein, AI173248 Protein, AU019541 Protein, BB114024 Protein, mRbAp46 Protein, rbap46 Protein, RBBP7 Protein, RBBP-7 Protein, Caf1 Protein, rbbp7 Protein, wu:fa13g08 Protein, wu:fb50h10 Protein, wu:fc29d09 Protein, zgc:56477 Protein, zgc:85617 Protein, CaO19.2146 Protein, RB binding protein 7, chromatin remodeling factor Protein, retinoblastoma binding protein 7, chromatin remodeling factor Protein, retinoblastoma binding protein 7 L homeolog Protein, retinoblastoma binding protein 7 Protein, retinoblastoma binding protein 4, like Protein, histone-binding protein RBBP7 Protein, Hat2p Protein, RBBP7 Protein, Rbbp7 Protein, rbbp7.L Protein, rbbp7 Protein, rbb4l Protein, LOC108708306 Protein, HAT2 Protein

Sujet: Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication, the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression, the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling, and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development, and the NURF (nucleosome remodeling factor) complex. {ECO:0000269|PubMed:10866654}.

Poids moléculaire: 48.6 kDa Including tag.

UniProt: Q16576