FAM115A Protein (AA 1-921) (Strep Tag)

N° du produit ABIN3095836

Détail du produit

Antigène: FAM115A (Family with Sequence Similarity 115, Member A (FAM115A))

Type de proteíne: Recombinant

Attributs du protein: AA 1-921

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette FAM115A protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MATPSAAFEA LMNGVTSWDV PEDAVPCELL LIGEASFPVM VNDMGQVLIA ASSYGRGRLV VVSHEDYLVE AQLTPFLLNA VGWLCSSPGA PIGVHPSLAP LAKILEGSGV DAKVEPEVKD SLGVYCIDAY NETMTEKLVK FMKCGGGLLI GGQAWDWANQ GEDERVLFTF PGNLVTSVAG IYFTDNKGDT SFFKVSKKMP KIPVLVSCED DLSDDREELL HGISELDISN SDCFPSQLLV HGALAFPLGL DSYHGCVIAA ARYGRGRVVV TGHKVLFTVG KLGPFLLNAV RWLDGGRRGK VVVQTELRTL SGLLAVGGID TSIEPNLTSD ASVYCFEPVS EVGVKELQEF VAEGGGLFVG AQAWWWAFKN PGVSPLARFP GNLLLNPFGI SITSQSLNPG PFRTPKAGIR TYHFRSTLAE FQVIMGRKRG NVEKGWLAKL GPDGAAFLQI PAEEIPAYMS VHRLLRKLLS RYRLPVATRE NPVINDCCRG AMLSLATGLA HSGSDLSLLV PEIEDMYSSP YLRPSESPIT VEVNCTNPGT RYCWMSTGLY IPGRQIIEVS LPEAAASADL KIQIGCHTDD LTRASKLFRG PLVINRCCLD KPTKSITCLW GGLLYIIVPQ NSKLGSVPVT VKGAVHAPYY KLGETTLEEW KRRIQENPGP WGELATDNII LTVPTANLRT LENPEPLLRL WDEVMQAVAR LGAEPFPLRL PQRIVADVQI SVGWMHAGYP IMCHLESVQE LINEKLIRTK GLWGPVHELG RNQQRQEWEF PPHTTEATCN LWCVYVHETV LGIPRSRANI ALWPPVREKR VRIYLSKGPN VKNWNAWTAL ETYLQLQEAF GWEPFIRLFT EYRNQTNLPT ENVDKMNLWV KMFSHQVQKN LAPFFEAWAW PIQKEVATSL AYLPEWKENI MKLYLLTQMP H
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FAM115A (Family with Sequence Similarity 115, Member A (FAM115A))

Autre désignation: TCAF1 (FAM115A Produits)

Synonymes: 2810407D09Rik Protein, 3321401G04Rik Protein, A230020K05Rik Protein, AI848529 Protein, TRPM8 channel associated factor 1 Protein, TRPM8 channel-associated factor 1 Protein, TCAF1 Protein, Tcaf1 Protein

Sujet: TRPM8 channel-associated factor 1 (TRP channel-associated factor 1),FUNCTION: Positively regulates the plasma membrane cation channel TRPM8 activity. Involved in the recruitment of TRPM8 to the cell surface. Promotes prostate cancer cell migration inhibition in a TRPM8-dependent manner. {ECO:0000269|PubMed:25559186}.

Poids moléculaire: 102.1 kDa

UniProt: Q9Y4C2