USP32 Protein (AA 1-1601) (His tag)

N° du produit ABIN3096130

Détail du produit

Antigène: USP32 (Ubiquitin Specific Peptidase 32 (USP32))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1601

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette USP32 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MGAKESRIGF LSYEEALRRV TDVELKRLKD AFKRTCGLSY YMGQHCFIRE VLGDGVPPKV AEVIYCSFGG TSKGLHFNNL IVGLVLLTRG KDEEKAKYIF SLFSSESGNY VIREEMERML HVVDGKVPDT LRKCFSEGEK VNYEKFRNWL FLNKDAFTFS RWLLSGGVYV TLTDDSDTPT FYQTLAGVTH LEESDIIDLE KRYWLLKAQS RTGRFDLETF GPLVSPPIRP SLSEGLFNAF DENRDNHIDF KEISCGLSAC CRGPLAERQK FCFKVFDVDR DGVLSRVELR DMVVALLEVW KDNRTDDIPE LHMDLSDIVE GILNAHDTTK MGHLTLEDYQ IWSVKNVLAN EFLNLLFQVC HIVLGLRPAT PEEEGQIIRG WLERESRYGL QAGHNWFIIS MQWWQQWKEY VKYDANPVVI EPSSVLNGGK YSFGTAAHPM EQVEDRIGSS LSYVNTTEEK FSDNISTASE ASETAGSGFL YSATPGADVC FARQHNTSDN NNQCLLGANG NILLHLNPQK PGAIDNQPLV TQEPVKATSL TLEGGRLKRT PQLIHGRDYE MVPEPVWRAL YHWYGANLAL PRPVIKNSKT DIPELELFPR YLLFLRQQPA TRTQQSNIWV NMGNVPSPNA PLKRVLAYTG CFSRMQTIKE IHEYLSQRLR IKEEDMRLWL YNSENYLTLL DDEDHKLEYL KIQDEQHLVI EVRNKDMSWP EEMSFIANSS KIDRHKVPTE KGATGLSNLG NTCFMNSSIQ CVSNTQPLTQ YFISGRHLYE LNRTNPIGMK GHMAKCYGDL VQELWSGTQK NVAPLKLRWT IAKYAPRFNG FQQQDSQELL AFLLDGLHED LNRVHEKPYV ELKDSDGRPD WEVAAEAWDN HLRRNRSIVV DLFHGQLRSQ VKCKTCGHIS VRFDPFNFLS LPLPMDSYMH LEITVIKLDG TTPVRYGLRL NMDEKYTGLK KQLSDLCGLN SEQILLAEVH GSNIKNFPQD NQKVRLSVSG FLCAFEIPVP VSPISASSPT QTDFSSSPST NEMFTLTTNG DLPRPIFIPN GMPNTVVPCG TEKNFTNGMV NGHMPSLPDS PFTGYIIAVH RKMMRTELYF LSSQKNRPSL FGMPLIVPCT VHTRKKDLYD AVWIQVSRLA SPLPPQEASN HAQDCDDSMG YQYPFTLRVV QKDGNSCAWC PWYRFCRGCK IDCGEDRAFI GNAYIAVDWD PTALHLRYQT SQERVVDEHE SVEQSRRAQA EPINLDSCLR AFTSEEELGE NEMYYCSKCK THCLATKKLD LWRLPPILII HLKRFQFVNG RWIKSQKIVK FPRESFDPSA FLVPRDPALC QHKPLTPQGD ELSEPRILAR EVKKVDAQSS AGEEDVLLSK SPSSLSANII SSPKGSPSSS RKSGTSCPSS KNSSPNSSPR TLGRSKGRLR LPQIGSKNKL SSSKENLDAS KENGAGQICE LADALSRGHV LGGSQPELVT PQDHEVALAN GFLYEHEACG NGYSNGQLGN HSEEDSTDDQ REDTRIKPIY NLYAISCHSG ILGGGHYVTY AKNPNCKWYC YNDSSCKELH PDEIDTDSAY ILFYEQQGID YAQFLPKTDG KKMADTSSMD EDFESDYKKY C
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human USP32 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: USP32 (Ubiquitin Specific Peptidase 32 (USP32))

Autre désignation: USP32 (USP32 Produits)

Synonymes: NY-REN-60 Protein, USP10 Protein, 2900074J03Rik Protein, 6430526O11Rik Protein, AW045245 Protein, USP32 Protein, ubiquitin specific peptidase 32 Protein, ubiquitin carboxyl-terminal hydrolase 32 Protein, USP32 Protein, Usp32 Protein, usp32 Protein, LOC100167978 Protein, LOC100512129 Protein

Poids moléculaire: 182.3 kDa Including tag.

UniProt: Q8NFA0