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VMA21 Protein (AA 1-101) (Strep Tag)

VMA21 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). WB, ELISA, SDS
N° du produit ABIN3100515
  • Antigène Tous les produits VMA21
    VMA21 (Vacuolar H+-ATPase Homolog (VMA21))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-101
    Origine
    Humain
    Source
    • 1
    Tobacco (Nicotiana tabacum)
    Purification/Conjugué
    Cette VMA21 protéine est marqué à la Strep Tag.
    Application
    Western Blotting (WB), ELISA, SDS-PAGE (SDS)
    Séquence
    MERPDKAALN ALQPPEFRNE SSLASTLKTL LFFTALMITV PIGLYFTTKS YIFEGALGMS NRDSYFYAAI VAVVAVHVVL ALFVYVAWNE GSRQWREGKQ D
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Attributs du produit
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Pureté
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    VMA21 (Vacuolar H+-ATPase Homolog (VMA21))
    Autre désignation
    VMA21 (VMA21 Produits)
    Synonymes
    MEAX Protein, XMEA Protein, ATP6H Protein, ATP6V0E Protein, M9.2 Protein, Vma21 Protein, Vma21p Protein, RGD1566155 Protein, 2610030H06Rik Protein, AI840175 Protein, VMA21, vacuolar ATPase assembly factor Protein, ATPase H+ transporting V0 subunit e1 Protein, VMA21 vacuolar H+-ATPase homolog (S. cerevisiae) Protein, VMA21, vacuolar ATPase assembly factor L homeolog Protein, VMA21 Protein, ATP6V0E1 Protein, Vma21 Protein, vma21.L Protein
    Sujet
    Vacuolar ATPase assembly integral membrane protein VMA21 (Myopathy with excessive autophagy protein),FUNCTION: Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum. {ECO:0000255|HAMAP-Rule:MF_03058}.
    Poids moléculaire
    11.4 kDa
    UniProt
    Q3ZAQ7
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