AOC3 Protein (AA 2-763) (rho-1D4 tag)
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- Antigène Voir toutes AOC3 Protéines
- AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-763
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette AOC3 protéine est marqué à la rho-1D4 tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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NQKTILVLLI LAVITIFALV CVLLVGRGGD GGEPSQLPHC PSVSPSAQPW THPGQSQLFA DLSREELTAV MRFLTQRLGP GLVDAAQARP SDNCVFSVEL QLPPKAAALA HLDRGSPPPA REALAIVFFG RQPQPNVSEL VVGPLPHPSY MRDVTVERHG GPLPYHRRPV LFQEYLDIDQ MIFNRELPQA SGLLHHCCFY KHRGRNLVTM TTAPRGLQSG DRATWFGLYY NISGAGFFLH HVGLELLVNH KALDPARWTI QKVFYQGRYY DSLAQLEAQF EAGLVNVVLI PDNGTGGSWS LKSPVPPGPA PPLQFYPQGP RFSVQGSRVA SSLWTFSFGL GAFSGPRIFD VRFQGERLVY EISLQEALAI YGGNSPAAMT TRYVDGGFGM GKYTTPLTRG VDCPYLATYV DWHFLLESQA PKTIRDAFCV FEQNQGLPLR RHHSDLYSHY FGGLAETVLV VRSMSTLLNY DYVWDTVFHP SGAIEIRFYA TGYISSAFLF GATGKYGNQV SEHTLGTVHT HSAHFKVDLD VAGLENWVWA EDMVFVPMAV PWSPEHQLQR LQVTRKLLEM EEQAAFLVGS ATPRYLYLAS NHSNKWGHPR GYRIQMLSFA GEPLPQNSSM ARGFSWERYQ LAVTQRKEEE PSSSSVFNQN DPWAPTVDFS DFINNETIAG KDLVAWVTAG FLHIPHAEDI PNTVTVGNGV GFFLRPYNFF DEDPSFYSAD SIYFRGDQDA GACEVNPLAC LPQAAACAPD LPAFSHGGFS HN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human AOC3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin-free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product AOC3 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))
- Autre désignation
- AOC3 (AOC3 Produits)
- Synonymes
- HPAO Protein, SSAO Protein, VAP-1 Protein, VAP1 Protein, aoc2 Protein, BOLAO Protein, amine oxidase, copper containing 3 Protein, amine oxidase, copper containing 3 L homeolog Protein, AOC3 Protein, aoc3.L Protein, Aoc3 Protein
- Sujet
- Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis. {ECO:0000269|PubMed:17400359, ECO:0000269|PubMed:19588076, ECO:0000269|PubMed:23349812, ECO:0000269|PubMed:9653080}.
- Poids moléculaire
- 85.7 kDa Including tag.
- UniProt
- Q16853
- Pathways
- Feeding Behaviour
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