SYVN1 Protein (AA 23-617) (rho-1D4 tag)

N° du produit ABIN3116131

Détail du produit

Antigène: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Type de proteíne: Recombinant

Attributs du protein: AA 23-617

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette SYVN1 protéine est marqué à la rho-1D4 tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: YYLKHQFYPT VVYLTKSSPS MAVLYIQAFV LVFLLGKVMG KVFFGQLRAA EMEHLLERSW YAVTETCLAF TVFRDDFSPR FVALFTLLLF LKCFHWLAED RVDFMERSPN ISWLFHCRIV SLMFLLGILD FLFVSHAYHS ILTRGASVQL VFGFEYAILM TMVLTIFIKY VLHSVDLQSE NPWDNKAVYM LYTELFTGFI KVLLYMAFMT IMIKVHTFPL FAIRPMYLAM RQFKKAVTDA IMSRRAIRNM NTLYPDATPE ELQAMDNVCI ICREEMVTGA KRLPCNHIFH TSCLRSWFQR QQTCPTCRMD VLRASLPAQS PPPPEPADQG PPPAPHPPPL LPQPPNFPQG LLPPFPPGMF PLWPPMGPFP PVPPPPSSGE AVAPPSTSAA ALSRPSGAAT TTAAGTSATA ASATASGPGS GSAPEAGPAP GFPFPPPWMG MPLPPPFAFP PMPVPPAGFA GLTPEELRAL EGHERQHLEA RLQSLRNIHT LLDAAMLQIN QYLTVLASLG PPRPATSVNS TEETATTVVA AASSTSIPSS EATTPTPGAS PPAPEMERPP APESVGTEEM PEDGEPDAAE LRRRRLQKLE SPVAH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SYVN1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Autre désignation: SYVN1 (SYVN1 Produits)

Synonymes: DER3 Protein, HRD1 Protein, hrd1 Protein, 1200010C09Rik Protein, AW211966 Protein, C85322 Protein, D530017H19Rik Protein, Hrd1 Protein, RGD1310488 Protein, syvn1 Protein, syvn1-a Protein, syvn1-b Protein, wu:fk91f10 Protein, zgc:55735 Protein, zgc:77108 Protein, synoviolin 1 Protein, synovial apoptosis inhibitor 1, synoviolin Protein, synoviolin 1 S homeolog Protein, SYVN1 Protein, syvn1 Protein, Syvn1 Protein, syvn1.S Protein

Sujet: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. {ECO:0000269|PubMed:12459480, ECO:0000269|PubMed:12646171, ECO:0000269|PubMed:12975321, ECO:0000269|PubMed:14593114, ECO:0000269|PubMed:16289116, ECO:0000269|PubMed:16847254, ECO:0000269|PubMed:17059562, ECO:0000269|PubMed:17141218, ECO:0000269|PubMed:17170702, ECO:0000269|PubMed:22607976}.

Poids moléculaire: 66.7 kDa Including tag.

UniProt: Q86TM6

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling