LMTK3 Protein (AA 21-1460) (rho-1D4 tag)

N° du produit ABIN3117509

Détail du produit

Antigène: LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))

Type de proteíne: Recombinant

Attributs du protein: AA 21-1460

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette LMTK3 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: SPAHPDGFAL GRAPLAPPYA VVLISCSGLL AFIFLLLTCL CCKRGDVGFK EFENPEGEDC SGEYTPPAEE TSSSQSLPDV YILPLAEVSL PMPAPQPSHS DMTTPLGLSR QHLSYLQEIG SGWFGKVILG EIFSDYTPAQ VVVKELRASA GPLEQRKFIS EAQPYRSLQH PNVLQCLGLC VETLPFLLIM EFCQLGDLKR YLRAQRPPEG LSPELPPRDL RTLQRMGLEI ARGLAHLHSH NYVHSDLALR NCLLTSDLTV RIGDYGLAHS NYKEDYYLTP ERLWIPLRWA APELLGELHG TFMVVDQSRE SNIWSLGVTL WELFEFGAQP YRHLSDEEVL AFVVRQQHVK LARPRLKLPY ADYWYDILQS CWRPPAQRPS ASDLQLQLTY LLSERPPRPP PPPPPPRDGP FPWPWPPAHS APRPGTLSSP FPLLDGFPGA DPDDVLTVTE SSRGLNLECL WEKARRGAGR GGGAPAWQPA SAPPAPHANP SNPFYEALST PSVLPVISAR SPSVSSEYYI RLEEHGSPPE PLFPNDWDPL DPGVPAPQAP QAPSEVPQLV SETWASPLFP APRPFPAQSS ASGSFLLSGW DPEGRGAGET LAGDPAEVLG ERGTAPWVEE EEEEEEGSSP GEDSSSLGGG PSRRGPLPCP LCSREGACSC LPLERGDAVA GWGGHPALGC PHPPEDDSSL RAERGSLADL PMAPPASAPP EFLDPLMGAA APQYPGRGPP PAPPPPPPPP RAPADPAASP DPPSAVASPG SGLSSPGPKP GDSGYETETP FSPEGAFPGG GAAEEEGVPR PRAPPEPPDP GAPRPPPDPG PLPLPGPREK PTFVVQVSTE QLLMSLREDV TRNLLGEKGA TARETGPRKA GRGPGNREKV PGLNRDPTVL GNGKQAPSLS LPVNGVTVLE NGDQRAPGIE EKAAENGALG SPEREEKVLE NGELTPPRRE EKALENGELR SPEAGEKVLV NGGLTPPKSE DKVSENGGLR FPRNTERPPE TGPWRAPGPW EKTPESWGPA PTIGEPAPET SLERAPAPSA VVSSRNGGET APGPLGPAPK NGTLEPGTER RAPETGGAPR APGAGRLDLG SGGRAPVGTG TAPGGGPGSG VDAKAGWVDN TRPQPPPPPL PPPPEAQPRR LEPAPPRARP EVAPEGEPGA PDSRAGGDTA LSGDGDPPKP ERKGPEMPRL FLDLGPPQGN SEQIKARLSR LSLALPPLTL TPFPGPGPRR PPWEGADAGA AGGEAGGAGA PGPAEEDGED EDEDEEEDEE AAAPGAAAGP RGPGRARAAP VPVVVSSADA DAARPLRGLL KSPRGADEPE DSELERKRKM VSFHGDVTVY LFDQETPTNE LSVQAPPEGD TDPSTPPAPP TPPHPATPGD GFPSNDSGFG GSFEWAEDFP LLPPPGPPLC FSRFSVSPAL ETPGPPARAP DARPAGPVEN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LMTK3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))

Autre désignation: LMTK3 (LMTK3 Produits)

Synonymes: LMTK3 Protein, LMR3 Protein, TYKLM3 Protein, BC059845 Protein, aatyk3 Protein, lemur tyrosine kinase 3 Protein, lmtk3 Protein, LMTK3 Protein, Lmtk3 Protein

Sujet: Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (PubMed:21602804). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons (By similarity). {ECO:0000250|UniProtKB:Q5XJV6, ECO:0000269|PubMed:21602804}.

Poids moléculaire: 153.0 kDa Including tag.

UniProt: Q96Q04