LMTK3 Protein (AA 133-411) (His tag)

N° du produit ABIN6239743

Détail du produit

Antigène: LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))

Activité biologique: Active

Attributs du protein: AA 133-411

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette LMTK3 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.

Information d'application

Indications d'application: Isoelectric Point: 6.3

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))

Abstract: LMTK3 Produits

Synonymes: LMTK3 Protein, LMR3 Protein, TYKLM3 Protein, BC059845 Protein, aatyk3 Protein, lemur tyrosine kinase 3 Protein, lmtk3 Protein, LMTK3 Protein, Lmtk3 Protein

Sujet: Alternative Names: LMR3, TYKLM3, Serine/threonine-protein kinase LMTK3

Poids moléculaire: 46kDa

UniProt: Q96Q04