PCDHA4 Protein (AA 30-947) (rho-1D4 tag)
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- Antigène Voir toutes PCDHA4 Protéines
- PCDHA4 (Protocadherin alpha 4 (PCDHA4))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 30-947
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette PCDHA4 protéine est marqué à la rho-1D4 tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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QLHYSVSEEA KHGTFVGRIA QDLGLELAEL VPRLFRVASK GRGGLLEVNL QNGILFVNSR IDREELCRRS AECSIHLEVI VDRPLQVFHV DVEVRDINDN PPVFPATQKN LSIAESRPLD SRFPLEGASD ADIGENALLT YRLSPNEYFS LEKPPDDELV KGLGLILRKS LDREEAPEIF LVLTATDGGK PELTGTVQLL ITVLDANDNA PAFDRTIYKV RLLENVPNGT LVIKLNASDL DEGLNGDIVY SFSNDISPNV KSKFHIDPIT GQIIVKGYID FEESKSYEII VEGIDKGQLP LSGHCRVIVE VEDNNDNVPD LEFKSLSLPI REDAPLGTVI ALISVSDKDM GVNGLVTCSL TSHVPFKLVS TFKNYYSLVL DSALDRESVS AYELVVTARD GGSPSLWATA SVSVEVADVN DNAPAFAQPE YTVFVKENNP PGCHIFTVSA WDADAQENAL VSYSLVERRV GERALSSYVS VHAESGKVYA LQPLDHEELE LLQFQVTARD AGVPPLGSNV TLQVFVLDEN DNAPALLAPR AGGTGGAVSE LVPWSVGVGH VVAKVRAVDA DSGYNAWLSY ELQPGTGGAR IPFRVGLYTG EISTTRALDE TDAPRHRLLV LVKDHGEPAL TATATVLVSL VESGQAPKAS SRALVGAVGP DAALVDVNVY LIIAICAVSS LLVLTLLLYT ALRCSALPTE GACAPGKPTL VCSSAVGSWS YSQQRRPRVC SGEGPPKTDL MAFSPSLPDS RDREDQLQTT EESFAKPRQP NPDWRYSASL RAGMHSSVHL EEAGILRAGP GGPDQQWPTV SSATPEPEAG EVSPPVGAGV NSNSWTFKYG PGNPKQSGPG ELPDKFIIPG SPAIISIRQE PTNSQIDKSD FITFGKKEET KKKKKKKKGN KTQEKKEKGN STTDNSDQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PCDHA4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin-free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product PCDHA4 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PCDHA4 (Protocadherin alpha 4 (PCDHA4))
- Autre désignation
- PCDHA4 (PCDHA4 Produits)
- Synonymes
- CNR1 Protein, CNRN1 Protein, CRNR1 Protein, PCDH-ALPHA4 Protein, Cnr1 Protein, Crnr1 Protein, R75250 Protein, PCDHA4 Protein, CNRv4 Protein, Pcdha4 Protein, protocadherin alpha 4 Protein, protocadherin alpha-4 Protein, PCDHA4 Protein, Pcdha4 Protein, LOC100713594 Protein, LOC100629843 Protein
- Sujet
- Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
- Poids moléculaire
- 100.3 kDa Including tag.
- UniProt
- Q9UN74
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