PLA2G4E Protein (AA 1-875) (Strep Tag)

N° du produit ABIN3122393

Détail du produit

Antigène: PLA2G4E (Phospholipase A2, Group IVE (PLA2G4E))

Type de proteíne: Recombinant

Attributs du protein: AA 1-875

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PLA2G4E protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MQSIPHSDEA DVAGMTHASE GHHGLGTSML VPKNPQGEED SKLGRNCSGF EDAQDPQTAV PSSPLLSMAS CSSQEGSSPC HLLTVRIIGM KNVRQADILS QTDCFVTLWL PTASQKKLKT RTISNCLHPE WDESFTFQIQ TQVKNVLELS VCDEDTLTQN DHLLTVLYDL SKLCLRNKTH VKFPLNPEGM EELEVEFLLE ENFSSSETLI TNGVLVSRQV SCLEVHAESR RPRKRKKNKD LLVMVTDSFE NTQRVPPCQE PCYPNSACFH YPKYSQPQLY AEAPKSHCNF RLCCCGTHRN DPVCQPLNCL SDGQVTTLPV GENYELHMKS SPCSDTLDVR LGFSLCQEEV EFVQKRKMVV AKTLSQMLQL EEGLHEDEVP IIAIMATGGG TRSMVSLYGH LLGLQKLNFL DASTYITGLS GATWTMATLY SDPEWSSKNL ETVVFEARRH VVKDKMPALF PDQLYKWRED LQKHSQEGYK TTFTDFWGKL IEYSLGDKKN ECKLSDQRAA LCRGQNPLPI YLTINVKDDV SNQDFREWFE FSPYEVGMQK YGAFIPSELF GSEFFMGRLM KRIPEPEMCY MLGLWSSIFS LNLLDAWNLS HTSEEFFYRW TRERLHDIED DPILPEIPRC DDNPLETTVV IPTTWLSNTF REILTRRPFV SEFHNFLYGM QLHTDYLQNR QFSMWKDTVL DTFPNQLTQF AKHLNLLDTA FFVNSSYAPL LRPERKVDLI IHLNYCAGSQ TKPLKQTCEY CTEQKIPFPS FSILEDDNSL KECYVMENPQ EPDAPIVAYF PLISDTFQKY KAPGVERSPD ELELGQLNIY GPKSPYATKE LTYTEAAFDK LVKLSEYNIL NNRDKLIQAL RLAMEKKRMR SQCPS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PLA2G4E (Phospholipase A2, Group IVE (PLA2G4E))

Autre désignation: Pla2g4e (PLA2G4E Produits)

Synonymes: RGD1310595 Protein, 2310026J01Rik Protein, C230096D22 Protein, Pla2epsilon Protein, phospholipase A2, group IVE Protein, phospholipase A2 group IVE-like 6 Protein, phospholipase A2 group IVE Protein, Pla2g4e Protein, PLA2G4EL6 Protein, PLA2G4E Protein

Sujet: Cytosolic phospholipase A2 epsilon (cPLA2-epsilon) (EC 3.1.1.4) (Calcium-dependent N-acyltransferase) (Phospholipase A2 group IVE),FUNCTION: Calcium-dependent N-acyltransferase involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PubMed:27399000). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PubMed:27399000, PubMed:29447909). Has weak phospholipase A2 and lysophospholipase activities (PubMed:27399000, PubMed:15866882). Regulates intracellular membrane trafficking that requires modulation of membrane curvature as it occurs by enrichment in lysophospholipids. Promotes tubule formation involved in clathrin-independent endocytotic trafficking and cargo recycling (PubMed:24413173). {ECO:0000269|PubMed:15866882, ECO:0000269|PubMed:24413173, ECO:0000269|PubMed:27399000, ECO:0000269|PubMed:29447909}.

Poids moléculaire: 100.2 kDa

UniProt: Q50L42

Pathways: Inositol Metabolic Process, VEGF Signaling