ORC2 Protein (AA 1-576) (Strep Tag)

N° du produit ABIN3122769

Détail du produit

Antigène: ORC2 (Origin Recognition Complex Subunit 2 (ORC2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-576

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ORC2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSTLQLKETK VPSVQFVGDD DVLSHILDRE GGTKLKKEKA QLLVNPQKVI KKADCELEKS DLEVLEDQNY VKVLGRNIQE SLGNGSAKDG RNKVYSFQQR KHPEEMTKLA LELAKTSGKK DPLDSNDPEI TKNIAQKSKG HSTSEKAPLV NNNKTEFLST QPHNLRKRII ASRSHYDSES EYSASSSEDD EEATKDEEED TNVARLSQKS QGQNRLLPAP VSKETLPKKK KRDKASDLVE EYFEAHSSSK VLTSDRTLQR LRRARVDQKT LHNLLRKFVP SFSAEIERLN QQHEKLFHKW MLQLHLGFNI VLYGLGSKRD LLEKFRTTML QDSIHVVING YFPGVSVKSI LNSITEDVLS HVGTFQSVLD QRDWIINRFK EDSSLELFLL IHNLDSQMLR GDNSQQILGQ LSSLHNVYLI ASIDHLNAPL MWDHAKQSLY NWLWYETTTY SPYTEETSYE NSLLVKQSGS LPLSSLIHVL RSLTPNARGI FRLLMKFQLD NQDSPSYIGL SFQDFYQQCR EAFLVNSDLT LRAQLTEFRD HKLIRTKKGT DGVEYLLIPV DSGILADFLE KEEEEA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ORC2 (Origin Recognition Complex Subunit 2 (ORC2))

Autre désignation: Orc2 (ORC2 Produits)

Synonymes: CG3041 Protein, DmORC 2 Protein, DmORC2 Protein, DmOrc2 Protein, DmOrc2p Protein, Dmel\\CG3041 Protein, ORC Protein, ORC2 Protein, Orc-2 Protein, Orc2p Protein, fs(3)293 Protein, fs(3)293-19 Protein, k43 Protein, l(3)88Ab Protein, l(3)A5 Protein, l(3)K43 Protein, l(3)SG44 Protein, l(3)dsl5 Protein, l(3)k43 Protein, orc2 Protein, rDmORC Protein, ATORC2 Protein, F13M22.6 Protein, F13M22_6 Protein, ORIGIN RECOGNITION COMPLEX SECOND LARGEST SUBUNIT 2 Protein, origin recognition complex second largest subunit 2 Protein, ORC2L Protein, AU041563 Protein, Orc2l Protein, orc2-a Protein, orc2l Protein, xorc2 Protein, Origin recognition complex subunit 2 Protein, origin recognition complex second largest subunit 2 Protein, origin recognition complex subunit 2 Protein, origin recognition complex, subunit 2 Protein, origin recognition complex subunit 2 S homeolog Protein, Orc2 Protein, ORC2 Protein, orc2.S Protein, orc-2 Protein

Sujet: Origin recognition complex subunit 2,FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 (By similarity). {ECO:0000250}.

Poids moléculaire: 65.9 kDa

UniProt: Q60862