Keratin 33B Protein (KRT33B) (AA 1-404) (Strep Tag)

N° du produit ABIN3123093

Détail du produit

Antigène: Keratin 33B (KRT33B)

Type de proteíne: Recombinant

Attributs du protein: AA 1-404

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Keratin 33B protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MPYNCCLPAM SCRTSCSSRP CVPPSCHGCT LPGACNIPAN VGNCNWFCEG SFNGNERETM QFLNDRLASY MEKVRQLERE NAELECKIQE RNQQQDPLVC PAYQAYFRTI EELQQKILCG KSENGRLVVQ IDNAKLAADD FRTKYESELS LRQLVESDLN GLRRILDELT LCKSDLEAQV ESLRQELLCL KQNHEEEVNT LRCQLGDRLN VEVDAAPTVD LNRVLNETRC QYEALVETNR REVEEWFTTQ TEELNKQVVS SSEQLQSCQA EIIELRRTVN ALEIELQAQH SMRNSLENTL AESEARYSSQ LSQVQCLITN VESQLGEIRA DLERQNQEYQ VLLDVKARLE CEINTYRGLL ESEDCKLPCN PCATTNACDK PIGPCVTNPC VTRSRCGPCN SFGC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Keratin 33B (KRT33B)

Autre désignation: Krt33b (KRT33B Produits)

Synonymes: Ka28 Protein, KRT33A Protein, KRT33B Protein, HA3II Protein, Ha-3II Protein, K33B Protein, KRTHA3A Protein, KRTHA3B Protein, hHa3-II Protein, Ha3 Protein, Ha4 Protein, Krt1-3 Protein, mHa3 Protein, keratin 33B Protein, keratin, type I cuticular Ha3-II Protein, keratin, type I cuticular Ha1 Protein, keratin, type I cuticular Ha3-I-like Protein, Krt33b Protein, KRT33B Protein, LOC705939 Protein, LOC100442854 Protein, LOC100526782 Protein, LOC618455 Protein, LOC100516036 Protein

Sujet: Keratin, type I cuticular Ha3-II (Hair keratin, type I Ha3) (Keratin, type I cuticular Ha3) (Keratin-33B) (K33B)

Poids moléculaire: 45.9 kDa

UniProt: Q61897