ATP7A Protein (AA 1003-1347) (His tag)

N° du produit ABIN3123303

Détail du produit

Antigène: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Type de proteíne: Recombinant

Attributs du protein: AA 1003-1347

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette ATP7A protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: VMVGTGVGAQ NGILIKGGEP LEMAHKVKVV VFDKTGTITH GTPVVNQVKV LVESNKISRN KILAIVGTAE SNSEHPLGAA VTKYCKKELD TETLGTCTDF QVVPGCGISC KVTNIEGLLH KSNLKIEENN IKNASLVQID AINEQSSTSS SMIIDAHLSN AVNTQQYKVL IGNREWMIRN GLVISNDVDE SMIEHERRGR TAVLVTIDDE LCGLIAIADT VKPEAELAVH ILKSMGLEVV LMTGDNSKTA RSIASQVGIT KVFAEVLPSH KVAKVKQLQE EGKRVAMVGD GINDSPALAM ANVGIAIGTG TDVAIEAADV VLIRNDLLDV VASIDLSRKT VKRIR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Atp7a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Autre désignation: Atp7a (ATP7A Produits)

Synonymes: ATP7A Protein, cal Protein, wu:fc43e01 Protein, zgc:153422 Protein, zgc:158633 Protein, DDBDRAFT_0218568 Protein, DDBDRAFT_0235190 Protein, DDB_0218568 Protein, DDB_0235190 Protein, atpase Protein, Atp7a Protein, kal Protein, atp7a Protein, DSMAX Protein, MK Protein, MNK Protein, SMAX3 Protein, Blo Protein, DXHXS1608e Protein, I14 Protein, Mo Protein, blotchy Protein, br Protein, brindled Protein, mottled Protein, Mnk Protein, ATPase copper transporting alpha Protein, ATPase, Cu++ transporting, alpha polypeptide Protein, P-type ATPase Protein, ATP synthase subunit a Protein, copper-transporting ATPase 1 Protein, ATP7A Protein, atp7a Protein, LOC100049514 Protein, Atp7a Protein, LOC412379 Protein

Sujet: May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells (By similarity). {ECO:0000250}.

Poids moléculaire: 37.8 kDa Including tag.

UniProt: Q64430

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process