Microrchidia 2A Protein (MORC2A) (AA 1-1030) (Strep Tag)

N° du produit ABIN3123434

Détail du produit

Antigène: Microrchidia 2A (MORC2A)

Type de proteíne: Recombinant

Attributs du protein: AA 1-1030

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Microrchidia 2A protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MAFTNYSSLN RAQLTFEYLH TNSTTHEFLF GALAELVDNA RDADATRIDI YAERREDLRG GFMLCFLDDG AGMDPSDAAS VIQFGKSAKR TPESTQIGQY GNGLKSGSMR IGKDFILFTK KEDTMTCLFL SRTFHEEEGI DEVIVPLPTW NARTREPITD NVEKFAIETE LVYKYSPFHT EEQVMNQFMK IPGNSGTLVI IFNLKLMDNG EPELDIISNP KDIQMAETSP EGTKPERRSF RAYAAVLYID PRMRIFIHGH KVQTKRLSCC LYKPRMYKYT SSRFKTRAEQ EVKKAEHVAR IAEEKAREAE SKARTLEVRM GGDLTRDSRV MLRQVQNTAI TLRREADVKK RIKDAKQRAL KEPKELNFVF GVNIEHRDLD GMFIYNCSRL IKMYEKVGPQ LEGGMACGGV VGVVDVPYLV LEPTHNKQDF ADAKEYRHLL RAMGEHLAQY WKDIAIAQRG IIKFWDEFGY LSANWNQPPS SELRFKRRRA MEIPTTIQCD LCLKWRTLPF QLSSVETDYP DTWVCSMNPD PEQDRCEASE QKQKVPLGTL KKDPKTQEEK QKQLTEKIRQ QQEKLEALQK TTPIRSQADL KKLPLEVTTR PIEEPVRRPQ RPRSPPLPAV IKNAPSRPPS IQTPRPSTQL RKTSVISLPK PPTTAARGET STSRLLQPTE APRKPANPPI KTVPRPTPPV HTPPLSLIPS SKSLREVPAQ KAIKTPVVKK PEPPVKQSVA TSGRKRSLAV SDEEEAEEEA EKRRERCKRG KLAVKEEKKE ANELSDSAGE DHPAELRKAQ KDKGLHVEVR VNREWYTGRV TAVEVGKNAV RWKVKFDYVP TDTTPRDRWV EKGSEDVRLM KPPSPEHQSP DTQQEGGEEE EAMVARQAVA LPEPSTSDGL PIEPDTTATS PSHETIDLLV QILRNCLRYF LPPSFPISKK ELSVMNSEEL ISFPLKEYFK QYEVGLQNLC HSYQSRADSR AKASEESLRT SEKKLRETEE KLQKLRTNIV ALLQKVQEDI DINTDDELDA YIEDLITKGD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Microrchidia 2A (MORC2A)

Autre désignation: Morc2a (MORC2A Produits)

Synonymes: 8430403M08Rik Protein, Zcwcc1 Protein, microrchidia 2A Protein, Morc2a Protein

Sujet: ATPase MORC2A (EC 3.6.1.-) (MORC family CW-type zinc finger protein 2A) (Zinc finger CW-type coiled-coil domain protein 1),FUNCTION: Essential for epigenetic silencing by the HUSH complex. Recruited by HUSH to target site in heterochromatin, the ATPase activity and homodimerization are critical for HUSH-mediated silencing (By similarity). Represses germ cell-related genes and L1 retrotransposons in collaboration with SETDB1 and the HUSH complex, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark (PubMed:29728365). Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. During DNA damage response, regulates chromatin remodeling through ATP hydrolysis (By similarity). During DNA damage response, may regulate chromatin remodeling through ATP hydrolysis (By similarity). {ECO:0000250|UniProtKB:Q9Y6X9, ECO:0000269|PubMed:29728365}.

Poids moléculaire: 117.3 kDa

UniProt: Q69ZX6