RBM26 Protein (AA 1-1012) (Strep Tag)

N° du produit ABIN3123574

Détail du produit

Antigène: RBM26 (RNA Binding Motif Protein 26 (RBM26))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1012

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette RBM26 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MVSKMIIENF EALKSWLSKT LEPICDADPS ALAKYVLALV KKDKSEKELK ALCIDQLDVF LQKETQIFVE KLFDAVNTKS YLPPPEQPSS GSLKVDFLQH QEKDIKKEEL TKEEEREKKF SRRLNHSPPQ SSSRYRDNRS RDERKKDDRS RKRDYDRNPP RRDSYRDRYN RRRGRSRSYS RSRSRSWSKE RLRDRDRDRS RTRSRSRTRS RERDLVKPKY DLDRTDPLEN NYTPVSSVSN ISSGHYPVPT LSSTITVIAP THHGNNTTES WSEFHEDQVD HNSYVRPPMP KKRCRDYDEK GFCMRGDMCP FDHGSDPVVV EDVNLPGMLP FPAQPPVVEG PPPPGLPPPP PILTPPPVNL RPPVPPPGPL PPSLPPVTGP PPPLPPLQPS GMDAPPNSAT SSVPTVVTTG IHHQPPPAPP SLFTAVFVLP DTYDTDGYNP EAPSITNTSR PMYRHRVHAQ RPNLIGLTSG DMDLPPREKP PNKSSMRIVV DSESRKRTIG SGEPGVSTKK TWFDKPNFNR TNSPGFQKKV QFGNENTKLE LRKVPPELNN ISKLNEHFSR FGTLVNLQVA YNGDPEGALI QFATYEEAKK AISSTEAVLN NRFIKVYWHR EGTTQQLQTT SPKVIQPLVQ QPILPVVKQS VKERLGPVPS ATTEPAEAQS ATSELPQNVT KLSVKDRLGF VSKPSVSATE KVLSTSTGLT KTVYNPAALK AAQKTLSVST PAVDNNEAQK KKQEALKLQQ DVRKRKQEIL EKHIETQKML ISKLEKNKTM KSEDKAEIMK TLEILTKNIT KLKDEVKSTS PGRCLPKSIK TKTQMQKELL DTELDLYKKM QAGEEVTELR RKYTELQLEA AKRGILSSGR GRGIHTRGRG TAHGRGRGRG RGRGVPGHAV VDHRPRALEI SAFTESDRED LLPHFAQYGE IEDCQIDDAS LHAIITFKTR AEAEAAAIHG ARFKGQDLKL AWNKPIANMS AVDTEEAEPD EEEFQEESLV DDSLLQDDDE EEEDNESRSW RR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RBM26 (RNA Binding Motif Protein 26 (RBM26))

Autre désignation: Rbm26 (RBM26 Produits)

Synonymes: rbm26 Protein, wu:fc66d05 Protein, DKFZp459J2451 Protein, ARRS2 Protein, C13orf10 Protein, RP11-255E21.1 Protein, SE70-2 Protein, ZC3H17 Protein, 1700009P03Rik Protein, AI447318 Protein, C230097K14Rik Protein, C86225 Protein, MLZ-393 Protein, Pro1777 Protein, Se70-2 Protein, Rbm26 Protein, RNA binding motif protein 26 Protein, cutaneous T-cell lymphoma tumor antigen se70-2 Protein, RNA binding motif protein 26 S homeolog Protein, RBM26 Protein, LOAG_02616 Protein, rbm26 Protein, Rbm26 Protein, rbm26.S Protein

Sujet: RNA-binding protein 26 (Protein expressed in male leptotene and zygotene spermatocytes 393) (MLZ-393) (RNA-binding motif protein 26),FUNCTION: May be involved in the turnover of nuclear polyadenylated (pA+) RNA. {ECO:0000250|UniProtKB:Q5T8P6}.

Poids moléculaire: 114.1 kDa

UniProt: Q6NZN0