UBOX5 Protein (AA 1-539) (Strep Tag)
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- Antigène Voir toutes UBOX5 Protéines
- UBOX5 (U-Box Domain Containing 5 (UBOX5))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-539
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette UBOX5 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MVVNLCLPQF RPRIHCNKVS ADGYEVENLI SEDLIKRSHG FRTEYFIRPP IYVTVSFPFN VEICRVNIDL TTGGYQNVSG LELYTSALSS RVSQDAQDCW TTGPVETSVP DKEAFTLVGK VLLKNQNHVV FSHRGFKARP PFSPMEVTLL SPAVVAQELW NKGALSLSHV AHLKIGITHV TGSGISCIKR LEVWGQPART CSQEVINSVL LIASESLPQD LDLHAPALPM ESDCDPGGQS ESQHSPCTLQ DMSEVESDVP EEFLDPITLE IMPCPMLLPS GKVIDQSTLE KCNLSEAAWG RVPSDPFTGL AFTPQSQPLP HPSLKARIDR FLLQHSISGC RLLGRAQTPS AMTPSVITLP SRKRKTEQAE HSSHYSLGMS ASSSATSPLF SPTTSEPTAK KMKATSELGL TDMDCSAGPV SHEQKLAQSL EIALTSTLGS MPSFTARLTK GQLQLGTRGS SACRRPASSS EHPRSVSGPE CASCKQAFSS YSTNEPVYQL PCGHLLCRPC LSEKQRSQPM MCTACRQPVT SQDVLRVHF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product UBOX5 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. - Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- 12 months
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- Antigène
- UBOX5 (U-Box Domain Containing 5 (UBOX5))
- Autre désignation
- Ubox5 (UBOX5 Produits)
- Synonymes
- RGD1305440 Protein, 1500010O06Rik Protein, C330018L13Rik Protein, Rnf37 Protein, UIP5 Protein, Ubce7ip5 Protein, Ube7ip5 Protein, RNF37 Protein, UBCE7IP5 Protein, U-box domain containing 5 Protein, U-box domain containing 5 S homeolog Protein, U box domain containing 5 Protein, Ubox5 Protein, UBOX5 Protein, ubox5.S Protein
- Sujet
- RING finger protein 37 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase RNF37) (U-box domain-containing protein 5) (UbcM4-interacting protein 5),FUNCTION: May have a ubiquitin-protein ligase activity acting as an E3 ubiquitin-protein ligase or as a ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. {ECO:0000269|PubMed:11435423}.
- Poids moléculaire
- 58.7 kDa
- UniProt
- Q925F4
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