YWHAB Protein (AA 2-246) (His tag)

N° du produit ABIN3128381

Détail du produit

Antigène: YWHAB (14-3-3 alpha + beta (YWHAB))

Type de proteíne: Recombinant

Attributs du protein: AA 2-246

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette YWHAB protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: TMDKSELVQK AKLAEQAERY DDMAAAMKAV TEQGHELSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT ERNEKKQQMG KEYREKIEAE LQDICNDVLE LLDKYLILNA TQAESKVFYL KMKGDYFRYL SEVASGENKQ TTVSNSQQAY QEAFEISKKE MQPTHPIRLG LALNFSVFYY EILNSPEKAC SLAKTAFDEA IAELDTLNEE SYKDSTLIMQ LLRDNLTLWT SENQGDEGDA GEGEN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ywhab Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: YWHAB (14-3-3 alpha + beta (YWHAB))

Autre désignation: Ywhab (YWHAB Produits)

Synonymes: GW128 Protein, HS1 Protein, KCIP-1 Protein, YWHAA Protein, 1300003C17Rik Protein, wu:fb22g04 Protein, wu:fr86a12 Protein, ywhab Protein, ywhab2 Protein, zgc:64092 Protein, 14-3-3beta Protein, fb80c08 Protein, fk41f08 Protein, wu:fb80c08 Protein, wu:fd11d01 Protein, wu:fk41f08 Protein, ywhab1 Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta S homeolog Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta L homeolog Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide a Protein, YWHAB Protein, Ywhab Protein, ywhabb Protein, ywhab.S Protein, ywhab.L Protein, ywhaba Protein

Sujet: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activatation of MAP kinases via AKAP13. {ECO:0000250|UniProtKB:P31946}.

Poids moléculaire: 28.9 kDa Including tag.

UniProt: Q9CQV8

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location