HAP1 Protein (AA 1-628) (Strep Tag)

N° du produit ABIN3131645

Détail du produit

Antigène: HAP1 (Huntingtin Associated Protein 1 (HAP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-628

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette HAP1 protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Séquence: MRPKEQVQSG AGDGTGSGDP AAGTPTTQPA VGPAPEPSAE PKPAPAQGTG SGQKSGSRTK TGSFCRSMII GDSDAPWTRY VFQGPYGPRA TGLGTGKAEG IWKTPAAYIG RRPGVSGPER AAFIRELQEA LCPNPPPTKK ITEDDVKVML YLLEEKERDL NTAARIGQSL VKQNSVLMEE NNKLETMLGS AREEILHLRK QVNLRDDLLQ LYSDSDDDDD EEDEEDEEEG EEEEREGQRD QDQQHDHPYG APKPHPKAET AHRCPQLETL QQKLRLLEEE NDHLREEASH LDNLEDEEQM LILECVEQFS EASQQMAELS EVLVLRLEGY ERQQKEITQL QAEITKLQQR CQSYGAQTEK LQQMLASEKG IHSESLRAGS YMQDYGSRPR DRQEDGKSHR QRSSMPAGSV THYGYSVPLD ALPSFPETLA EELRTSLRKF ITDPAYFMER RDTHCREGRK KEQRAMPPPP AQDLKPPEDF EAPEELVPEE ELGAIEEVGT AEDGQAEENE QASEETEAWE EVEPEVDETT RMNVVVSALE ASGLGPSHLD MKYVLQQLSN WQDAHSKRQQ KQKVVPKDSP TPQQQTNMGG GILEQQPRVP TQDSQRLEED RATHSPSARE EEGPSGAT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: HAP1 (Huntingtin Associated Protein 1 (HAP1))

Autre désignation: Hap1 (HAP1 Produits)

Synonymes: HAP2 Protein, HIP5 Protein, HLP Protein, hHLP1 Protein, HAP1-A Protein, HAP1-B Protein, HAP-1 Protein, huntingtin associated protein 1 Protein, huntingtin-associated protein 1 Protein, HAP1 Protein, Hap1 Protein

Sujet: Huntingtin-associated protein 1 (HAP-1),FUNCTION: Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion, the function seems to involve HTT, DCTN1 and a complex with SORT1. Involved in APP trafficking and seems to facilitate APP anterograde transport and membrane insertion thereby possibly reducing processing into amyloid beta. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptors to synapses, the function is dependent on kinesin motor protein KIF5 and is disrupted by HTT with expanded polyglutamine repeat. Involved in regulation of autophagosome motility by promoting efficient retrograde axonal transport. Seems to be involved in regulation of membrane receptor recycling and degradation, and respective signal transduction, including GABA(A) receptors, tyrosine kinase receptors, EGFR, IP3 receptor and androgen receptor. Among others suggested to be involved in control of feeding behavior (involving hypothalamic GABA(A) receptors), cerebellar and brainstem development (involving AHI1 and NTRK1/TrkA), postnatal neurogenesis (involving hypothalamic NTRK2/TrkB regulating the number of Npyr1-expressing cells), and ITPR1/InsP3R1-mediated Ca(2+) release (involving HTT and possibly the effect of mutant HTT). Via association with DCTN1/dynactin p150-glued and HTT/huntingtin involved in cytoplasmic retention of REST in neurons. May be involved in ciliogenesiss, however, reports are conflicting: PubMed:21985783 reports that Hap1 is required for ciliogenesis in primary cortical neurons and proposes that HTT interacts with PCM1 through HAP1, PubMed:23532844 reports that mice with disrupted Hap1 display normal cilium formation and function. Involved in regulation of exocytosis. Isoform A but not isoform B seems to be involved in formation of cytoplasmic inclusion bodies (STBs). In case of anomalous expression of TBP, can sequester a subset of TBP into STBs, sequestration is enhanced by an expanded polyglutamine repeat within TBP. {ECO:0000269|PubMed:12890790, ECO:0000269|PubMed:15379999, ECO:0000269|PubMed:17868456, ECO:0000269|PubMed:18636121, ECO:0000269|PubMed:21985783, ECO:0000269|PubMed:24355921, ECO:0000269|PubMed:24366265, ECO:0000269|PubMed:24453320}.

Poids moléculaire: 70.1 kDa

UniProt: O35668

Pathways: Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus