Fgr Protein (AA 2-517) (His tag)

N° du produit ABIN3132977

Détail du produit

Antigène: Fgr (FGR) (Gardner-Rasheed Feline Sarcoma Viral (V-Fgr) Oncogene Homolog (FGR))

Type de proteíne: Recombinant

Attributs du protein: AA 2-517

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette Fgr protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: GCVFCKKLEP ASKEDVGLEG DFRSQTAEER YFPDPTQGRT SSVFPQPTSP AFLNTGNMRS ISGTGVTIFV ALYDYEARTG DDLTFTKGEK FHILNNTEYD WWEARSLSSG HRGYVPSNYV APVDSIQAEE WYFGKISRKD AERQLLSSGN PQGAFLIRES ETTKGAYSLS IRDWDQNRGD HIKHYKIRKL DTGGYYITTR AQFDSIQDLV RHYMEVNDGL CYLLTAPCTT TKPQTLGLAK DAWEIDRNSI ALERRLGTGC FGDVWLGTWN CSTKVAVKTL KPGTMSPKAF LEEAQIMKLL RHDKLVQLYA VVSEEPIYIV TEFMCYGSLL DFLKDREGQN LMLPHLVDMA AQVAEGMAYM ERMNYIHRDL RAANILVGEY LICKIADFGL ARLIEDNEYN PQQGTKFPIK WTAPEAALFG RFTVKSDVWS FGILLTELIT KGRVPYPGMN NREVLEQVEH GYHMPCPPGC PASLYEVMEQ AWRLDPEERP TFEYLQSFLE DYFTSTEPQY QPGDQT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Fgr Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Fgr (FGR) (Gardner-Rasheed Feline Sarcoma Viral (V-Fgr) Oncogene Homolog (FGR))

Autre désignation: Fgr (FGR Produits)

Synonymes: SRC2 Protein, c-fgr Protein, c-src2 Protein, p55-Fgr Protein, p55c-fgr Protein, p58-Fgr Protein, p58c-fgr Protein, FGR proto-oncogene, Src family tyrosine kinase Protein, FGR Protein, Fgr Protein

Sujet: Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCER1G and FCGR2. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes (PubMed:11672534). Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages (PubMed:8666673 and PubMed:9687507). Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation (PubMed:15561106). Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling (PubMed:21746961). Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. {ECO:0000269|PubMed:10662797, ECO:0000269|PubMed:11672534, ECO:0000269|PubMed:15561106, ECO:0000269|PubMed:19903482, ECO:0000269|PubMed:21746961, ECO:0000269|PubMed:8125254, ECO:0000269|PubMed:8666673, ECO:0000269|PubMed:9687507}.

Poids moléculaire: 59.7 kDa Including tag.

UniProt: P14234

Pathways: Sensory Perception of Sound, Stem Cell Maintenance, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling