PTBP1 Protein (AA 1-527) (His tag)

N° du produit ABIN3133144

Détail du produit

Antigène: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-527

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PTBP1 protéine est marqué à la His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Séquence: MDGIVPDIAV GTKRGSDELF STCVSNGPFI MSSSASAANG NDSKKFKGDN RSAGVPSRVI HVRKLPSDVT EGEVISLGLP FGKVTNLLML KGKNQAFIEM NTEEAANTMV NYYTSVAPVL RGQPIYIQFS NHKELKTDSS PNQVRAQAAL QAVNSVQSGN LALAASAAAV DAGMAMAGQS PVLRIIVENL FYPVTLDVLH QIFSKFGTVL KIITFTKNNQ FQALLQYADP VSAQHAKLSL DGQNIYNACC TLRIDFSKLT SLNVKYNNDK SRDYTRPDLP SGDSQPSLDQ TMAAAFGLSV PNVHGALAPL AIPSAAAAAA ASRIAIPGLA GAGNSVLLVS NLNPERVTPQ SLFILFGVYG DVQRVKILFN KKENALVQMA DGSQAQLGEP PERAQAAREV SAHYTVQASE CAAAREGQED QGLTKDYGSS PLRFKKPGSK NFQNIFPPSA TLHLSNIPPS VSEDDLKSLF SSNGGVVKGF KFFQKDRKMA LIQMGSVEEA VQALIELHNH DLGENHHLRV SFSKSTI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ptbp1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Autre désignation: Ptbp1 (PTBP1 Produits)

Synonymes: HNRNP-I Protein, HNRNPI Protein, HNRPI Protein, PTB Protein, PTB-1 Protein, PTB-T Protein, PTB2 Protein, PTB3 Protein, PTB4 Protein, pPTB Protein, Ptb Protein, hnrpi Protein, hnrnp-I Protein, vgrbp60 Protein, AA407203 Protein, AL033359 Protein, Pybp Protein, Pybp1 Protein, Pybp2 Protein, ptbp1 Protein, wu:fb99f12 Protein, zgc:110689 Protein, ptb Protein, ATPTB1 Protein, POLYPYRIMIDINE TRACT-BINDING Protein, POLYPYRIMIDINE TRACT-BINDING PROTEIN 1 Protein, T4P13.16 Protein, T4P13_16 Protein, polypyrimidine tract-binding protein 1 Protein, PTBP1 Protein, polypyrimidine tract binding protein 1 Protein, polypyrimidine tract binding protein 1 S homeolog Protein, polypyrimidine tract-binding protein Protein, polypyrimidine tract binding protein 1a Protein, polypyrimidine tract binding protein 1 L homeolog Protein, polypyrimidine tract-binding protein 1 Protein, PTBP1 Protein, ptbp1.S Protein, NAEGRDRAFT_80143 Protein, LOC100285404 Protein, Ptbp1 Protein, ptbp1a Protein, ptbp1.L Protein, PTB1 Protein, LOC100061673 Protein

Sujet: Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10.

Poids moléculaire: 57.4 kDa Including tag.

UniProt: P17225

Pathways: Regulation of Muscle Cell Differentiation