Glutathione Reductase Protein (GSR) (AA 27-500) (His tag)

N° du produit ABIN3134142

Détail du produit

Antigène: Glutathione Reductase (GSR)

Type de proteíne: Recombinant

Attributs du protein: AA 27-500

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette Glutathione Reductase protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: MASPGEPQPP APDTSSFDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHVD YGFQSCEGKF SWHVIKQKRD AYVSRLNTIY QNNLTKSHIE IIHGYATFAD GPRPTVEVNG KKFTAPHILI ATGGVPTVPH ESQIPGASLG ITSDGFFQLE DLPSRSVIVG AGYIAVEIAG ILSALGSKTS LMIRHDKVLR NFDSLISSNC TEELENAGVE VLKFTQVKEV KKTSSGLELQ VVTSVPGRKP TTTMIPDVDC LLWAIGRDPN SKGLNLNKVG IQTDEKGHIL VDEFQNTNVK GVYAVGDVCG KALLTPVAIA AGRKLAHRLF ECKQDSKLDY DNIPTVVFSH PPIGTVGLTE DEAVHKYGKD NVKIYSTAFT PMYHAVTTRK TKCVMKMVCA NKEEKVVGIH MQGIGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Gsr Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Glutathione Reductase (GSR)

Autre désignation: Gsr (GSR Produits)

Synonymes: GR Protein, ATGR2 Protein, EMB2360 Protein, glutathione reductase Protein, AI325518 Protein, D8Ertd238e Protein, Gr-1 Protein, Gr1 Protein, DDBDRAFT_0168952 Protein, DDBDRAFT_0231410 Protein, DDB_0168952 Protein, DDB_0231410 Protein, 2151 Protein, CG2151 Protein, DTR Protein, Dm-TrxR Protein, DmTR Protein, DmTrx Protein, DmTrxR Protein, DmTrxR-1 Protein, Dmel\\CG2151 Protein, Gr Protein, Trx Protein, TrxR Protein, TrxR-1 Protein, Trxr1 Protein, anon-WO03040301.185 Protein, anon-WO03040301.187 Protein, dTrxR Protein, dmtrxr-1 Protein, gr Protein, l(1)G0154 Protein, l(1)G0379 Protein, l(1)G0477 Protein, l(1)G0481 Protein, trxr-1 Protein, gor1 Protein, glutathione reductase, gro-2 Protein, glutathione reductase Protein, glutathione-disulfide reductase Protein, GR; GRase Protein, mitochondrial glutathione reductase Pgr1 Protein, Glutathione reductase (GR) (GRase) Protein, Thioredoxin reductase-1 Protein, glutathione-disulfide reductase family protein Protein, glutathione reductase 1 Protein, GR Protein, gor Protein, GSR Protein, GSR1 Protein, Gsr_2 Protein, Gsr Protein, GSHR1 Protein, gsr Protein, LbGR Protein, pgr1 Protein, GSHR2 Protein, GLR2 Protein, Bcen_2392 Protein, RPE_1989 Protein, HCAG_02219 Protein, SJAG_01162 Protein, Trxr-1 Protein, PF14_0192 Protein, POPTR_0001s14480g Protein, gsr1 Protein

Sujet: Maintains high levels of reduced glutathione in the cytosol.

Poids moléculaire: 52.0 kDa Including tag.

UniProt: P47791

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis