GPC4 Protein (AA 19-529) (His tag)

N° du produit ABIN3134363

Détail du produit

Antigène: GPC4 (Glypican 4 (GPC4))

Type de proteíne: Recombinant

Attributs du protein: AA 19-529

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette GPC4 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: MKFLVNVALV FMVVYISYIY ASLLAAELKS KSCSEVRRLY VSKGFNKNDA PLYEINGDHL KICPQDYTCC SQEMEEKYSL QSKDDFKTVV SEQCNHLQAI FASRYKKFDE FFKELLENAE KSLNDMFVKT YGHLYMQNSE LFKDLFVELK RYYVAGNVNL EEMLNDFWAR LLERMFRLVN SQYHFTDEYL ECVSKYTEQL KPFGDVPRKL KLQVTRAFVA ARTFAQGLAV ARDVVSKVSV VNPTAQCTHA LLKMIYCSHC RGLVTVKPCY NYCSNIMRGC LANQGDLDFE WNNFIDAMLM VAERLEGPFN IESVMDPIDV KISDAIMNMQ DNSVQVSQKV FQGCGPPKPL PAGRISRSIS ESAFSARFRP YHPEQRPTTA AGTSLDRLVT DVKEKLKQAK KFWSSLPSTV CNDERMAAGN ENEDDCWNGK GKSRYLFAVT GNGLANQGNN PEVQVDTSKP DILILRQIMA LRVMTSKMKN AYNGNDVDFF DISDESSGEG SGSGCEYQQC PSEFEYNATD HSGKSANEKA DSGSSGHHHH HH

Specificité: N-terminal Melittin-Signalsequenz, C-terminal His-Tag

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Gpc4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 20 mM HEPES, pH 7.5; 150 mM NaCl, 10% Glycerol, 1mM DTT, 1mM EDTA)

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: GPC4 (Glypican 4 (GPC4))

Autre désignation: Gpc4 (GPC4 Produits)

Synonymes: K-glypican Protein, knypek Protein, xgly4 Protein, Xgly4 Protein, glypican-4 Protein, 9530073D23Rik Protein, AF311610 Protein, AI385680 Protein, AI661372 Protein, chunp6920 Protein, gpc4, Protein, gpc4/6 Protein, gpc6 Protein, kny Protein, wu:fc47a08 Protein, wu:fe05f10 Protein, glypican 4 Protein, glypican 4 L homeolog Protein, GPC4 Protein, gpc4.L Protein, gpc4 Protein, Gpc4 Protein

Sujet: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.

Poids moléculaire: 58.8 kDa Including tag.

UniProt: P51655

Pathways: Glycosaminoglycan Metabolic Process