MUSK Protein (AA 22-494) (His tag)

N° du produit ABIN3135335

Détail du produit

Antigène: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Type de proteíne: Recombinant

Attributs du protein: AA 22-494

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette MUSK protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: EKLPKAPVIT TPLETVDALV EEVATFMCAV ESYPQPEISW TRNKILIKLF DTRYSIRENG QLLTILSVED SDDGIYCCIA NNGVGGAVES CGALQVKMKP KITRPPINVK IIEGLKAVLP CTTMGNPKPS VSWIKGDNAL RENSRIAVLE SGSLRIHNVQ KEDAGQYRCV AKNSLGTAYS KLVKLEVEVF ARILRAPESH NVTFGSFVTL RCTAIGIPVP TISWIENGNA VSSGSIQESV KDRVIDSRLQ LFITKPGLYT CIATNKHGEK FSTAKAAATV SIAEWSKSQK DSQGYCAQYR GEVCDAVLAK DALVFFNTSY RDPEDAQELL IHTAWNELKA VSPLCRPAAE ALLCNHLFQE CSPGVVPTPM PICREYCLAV KELFCAKEWQ AMEGKAHRGL YRSGMHLLPV PECSKLPSMH RDPTACTRLP YLDYKKENIT TFPSITSSRP SADIPNLPAS TSSFAVSPAY SMT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Musk Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Autre désignation: Musk (MUSK Produits)

Synonymes: unp Protein, fj98e12 Protein, zgc:63467 Protein, wu:fj98e12 Protein, si:dz46m7.2 Protein, si:dz57f23.3 Protein, si:busm1-57f23.3 Protein, musk-A Protein, MuSK Protein, MUSK Protein, Mdk4 Protein, Mlk Protein, Nsk1 Protein, Nsk2 Protein, Nsk3 Protein, muscle Protein, muscle associated receptor tyrosine kinase Protein, muscle, skeletal, receptor tyrosine kinase Protein, muscle associated receptor tyrosine kinase S homeolog Protein, MUSK Protein, musk Protein, musk.S Protein, Musk Protein

Sujet: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation. {ECO:0000269|PubMed:11323662, ECO:0000269|PubMed:12756238, ECO:0000269|PubMed:14622576, ECO:0000269|PubMed:15340048, ECO:0000269|PubMed:8653786}.

Poids moléculaire: 52.9 kDa Including tag.

UniProt: Q61006

Pathways: Signalisation RTK, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development