NCOA2 Protein (AA 2-1462) (His tag)

N° du produit ABIN3135340

Détail du produit

Antigène: NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1462

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette NCOA2 protéine est marqué à la His tag.

Application: Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)

Séquence: SGMGENTSDP SRAETRKRKE CPDQLGPSPK RSTEKRNREQ ENKYIEELAE LIFANFNDID NFNFKPDKCA ILKETVKQIR QIKEQEKAAA ANIDEVQKSD VSSTGQGVID KDALGPMMLE ALDGFFFVVN LEGSVVFVSE NVTQYLRYNQ EELMNKSVYS ILHVGDHTEF VKNLLPKSMV NGGSWSGEPP RRSSHTFNCR MLVKPLPDSE EEGHDSQEAH QKYEAMQCFA VSQPKSIKEE GEDLQSCLIC VARRVPMKER PTLPSSESFT TRQDLQGKIT SLDTSTMRAA MKPGWEDLVR RCIQKFHTQH EGESLSYAKR HHHEVLRQGL AFSQIYRFSL SDGTLVAAQT KSKLIRSQTT NEPQLVISLH MLHREQNVCV MNPDLTGQAM GKPLNPISSS SPAHQALCSG NPGQDMTLGS NINFPMNGPK EQMGMPMGRF GGSGGMNHVS GMQATTPQGS NYALKMNSPS QSSPGMNPGQ ASSVLSPRQR MSPGVAGSPR IPPSQFSPAG SLHSPVGVCS STGNSHSYTN SSLNALQALS EGHGVSLGSS LASPDLKMGN LQNSPVNMNP PPLSKMGSLD SKDCFGLYGE PSEGTTGQAE ASCHPEEQKG PNDSSMPQAA SGDRAEGHSR LHDSKGQTKL LQLLTTKSDQ MEPSPLPSSL SDTNKDSTGS LPGPGSTHGT SLKEKHKILH RLLQDSSSPV DLAKLTAEAT GKELSQESSS TAPGSEVTVK QEPASPKKKE NALLRYLLDK DDTKDIGLPE ITPKLERLDS KTDPASNTKL IAMKTVKEEV SFEPSDQPGS ELDNLEEILD DLQNSQLPQL FPDTRPGAPT GSVDKQAIIN DLMQLTADSS PVPPAGAQKA ALRMSQSTFN NPRPGQLGRL LPNQNLPLDI TLQSPTGAGP FPPIRNSSPY SVIPQPGMMG NQGMLGSQGN LGNNSTGMIG SSTSRPSMPS GEWAPQSPAV RVTCAATTGA MNRPVQGGMI RNPTASIPMR ANSQPGQRQM LQSQVMNIGP SELEMNMGGP QYNQQQAPPN QTAPWPESIL PIDQASFASQ NRQPFGSSPD DLLCPHPAAE SPSDEGALLD QLYLALRNFD GLEEIDRALG IPELVSQSQA VDAEQFSSQE SSIMLEQKPP VFPQQYASQA QMAQGGYNPM QDPNFHTMGQ RPNYTTLRMQ PRPGLRPTGI VQNQPNQLRL QLQHRLQAQQ NRQPLMNQIS SVSNVNLTLR PGVPTQAPIN AQMLAQRQRE ILNQHLRQRQ MQQQVQQRTL MMRGQGLNVT PSMVAPAGLP AAMSNPRIPQ ANAQQFPFPP NYGISQQPDP GFTGATTPQS PLMSPRMAHT QSPMMQQSQA NPAYQPTSDM NGWAQGSMGG NSMFSQQSPP HFGQQANTSM YSNNMNISVS MATNTGGLSS MNQMTGQMSM TSVTSVPTSG LPSMGPEQVN DPALRGGNLF PNQLPGMDMI KQEGDASRKY C
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ncoa2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))

Autre désignation: Ncoa2 (NCOA2 Produits)

Synonymes: GRIP1 Protein, KAT13C Protein, NCoA-2 Protein, SRC2 Protein, TIF2 Protein, bHLHe75 Protein, 9530095N19 Protein, D1Ertd433e Protein, GRIP-1 Protein, Grip1 Protein, SRC-2 Protein, TIF-2 Protein, tif2 Protein, wu:fc41a10 Protein, wu:fe26a11 Protein, Tif2 Protein, NCOA2 Protein, XTIF2 Protein, bhlhe75 Protein, grip1 Protein, kat13c Protein, src2 Protein, ncoa2-A Protein, nuclear receptor coactivator 2 Protein, nuclear receptor coactivator 2 S homeolog Protein, NCOA2 Protein, Ncoa2 Protein, ncoa2 Protein, LOC100089041 Protein, ncoa2.S Protein

Sujet: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (PubMed:24529706). {ECO:0000269|PubMed:11997499, ECO:0000269|PubMed:12507421, ECO:0000269|PubMed:16148126, ECO:0000269|PubMed:19039140, ECO:0000269|PubMed:24529706}.

Poids moléculaire: 159.3 kDa Including tag.

UniProt: Q61026

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling