PPP1R13B Protein (AA 1-1087) (Strep Tag)

N° du produit ABIN3135552

Détail du produit

Antigène: PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1087

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PPP1R13B protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MMPMILTVFL SNNEQILTEV PITPETTCRD VVEFCKEPGE GGCHLAEVWR GSERPIPYDH MMYEHLQKWG PRREEVKFFL RHEDSPTESS EQGARQTQEQ RTQRSVVNVP GEKRTENGVG NPRVELTLSE LQDMAARQQQ QIENQQQMLV AKEQRLHFLK QQERRQQQSV SENEKLQKLK ERVEAQENKL KKIRAMRGQV DYSKIMNGNL SAEIERFSAM FQEKKQEVQT AILRVDQLSQ QLEDLKKGKL NGFQSYNGRL TGPAAVELKR LYQELQIRNQ LNQEQNSKLQ QQKELLNKRN MEVAMMDKRI SELRERLYGK KIQLNRVNGT SSPQSPLSTS GRVAAVGPYI QVPSTGGFPL PGDPVKPQSL TIASSAAHGR SKSANDGNWP PLKQNSASVK STQMTGDWKD SGMEGTLKQG AISSQPLPLS ALGATEKLGI EIGKGPPPIP GVGKPLPPSY GTYPSSGPLG PGSTSSLERR KEGSLPRPGA GPPSRQKPAP LPPASNAPQP GSSQQIQQRI SVPPSPTYPP AGPPAFPTGD GKPELPLTVA IRPFLADKGS RPQSPRKGPQ TVNSSSIYSM YLQQATPPKN YQPPAHGTLN KSVKAVYGKP VLPSGSASPS PLPFLHGSLG TGTAQPQPPS DSAEKEPEQE GPSVPGEGST VESLPRPLSP TKLTPIVHSP LRYQSDADLE ALRRKLANAP RPLKKRSSIT EPEGPGGPNI QKLLYQRFNT LAGGMEGTPF YQPSPSQDFV GTLADMDNGN TNANGNLDEP FPPRPTAPLP EELAPSSDAN DNELPSPEPE ELICPQTTHQ TAEPTEDNNN NVAPVPSTEQ IPSPVAEAPS EEDQVPPAPL SPVIHPPAAS ASKRTNLKKP NSERTGHGLR VRFNPLALLL DASLEGEFDL VQRIIYEVED PSKPNDEGIT PLHNAVCAGH HHIVKFLLDF GVNVNAADSD GWTPLHCAAS CNSVHLCKQL VESGAAIFAS TISDIETAAD KCEEMEEGYI QCSQFLYGVQ EKLGVMNKGT VYALWDYEAQ NSDELSFHEG DAITILRRKD ENETEWWWAR LGDREGYVPK NLLGLYPRIK PRQRTLA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))

Autre désignation: Ppp1r13b (PPP1R13B Produits)

Synonymes: ASPP1 Protein, p53BP2-like Protein, p85 Protein, AI449786 Protein, AW545810 Protein, Tp53bp2 Protein, Trp53bp2 Protein, protein phosphatase 1 regulatory subunit 13B Protein, protein phosphatase 1, regulatory subunit 13B Protein, protein phosphatase 1 regulatory subunit 13B L homeolog Protein, protein phosphatase 1, regulatory subunit 13Bb Protein, protein phosphatase 1, regulatory (inhibitor) subunit 13B Protein, PPP1R13B Protein, Ppp1r13b Protein, ppp1r13b.L Protein, ppp1r13bb Protein, ppp1r13b Protein

Sujet: Apoptosis-stimulating of p53 protein 1 (Protein phosphatase 1 regulatory subunit 13B),FUNCTION: Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53 (By similarity). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q96KQ4}.

Poids moléculaire: 119.2 kDa

UniProt: Q62415

Pathways: Signalisation p53