DHX57 Protein (AA 1-1388) (Strep Tag)

N° du produit ABIN3135751

Détail du produit

Antigène: DHX57 (DEAH (Asp-Glu-Ala-Asp/His) Box Polypeptide 57 (DHX57))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1388

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette DHX57 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MSSSVRRKGK PGKGDGKGSS RGGRGGKGHM NKSHGGGGGG GGSCGGGGGG SRKASNRIWD DGDDFCVFTE PKRPSRPCDS NKSKGETRPK WKPKAKVPLQ TLHMTSENQE KVKALLRDLQ EQGADAGSER GTSGEEEDSE PQCGEEQGWP AGQEPIFLPD CSPWEYIGPE EVEPPVPECA VSPLAVQKLS RYGFHTEHCQ LALRICDGDL GAALEHLLRQ CFSETFGERM ALSEAAVYVS LNECVEQRQE ETLALKSICG EKFIERIQNR VWTIGLELDY LTNKFCKSKQ KESSKNVRDT SPETCKFYLK GNCKFGSKCK FKHEVPPHQM IGRAERNVND PHLDADDDTT FMYELQIRFS KDHKYPYQAP LVAFYSTNEN LPLACRLHIS EFLYGKALEF AKTSEPVVYS LITLLEEESE IVKLLTHTQH KYSVPPVNVP PVPSETRISK PAYRKPVVPS NTFLSNQMLE GERLSELEED ADEDEGPASI IVENESYVNL KKRSYKRYDR PAKSLFAENS KICRQFQMKQ ASRQFHAILQ ERQLLPAWEE RETILKLLSK HQVVVISGMT GCGKTTQIPQ FILDNSLNGP PERVANIICT QPRRISAISV AERVAKERAE RVGLTVGYQI RLESVKSSAT RLLYCTTGVL LRRLEGDATL QGVTHIIVDE VHERTEESDF LLLVLKDIVM QRATLQVILM SATLDAGLFS KYFSYCPVIT IPGRAFPVDQ FFLEDALAVT RYVLQDGSPY MRSMKQIAKE KLKARHNRTA QEEVEEDLRL SLHLQDEEES VKDTIPDQQL DFKQLLIRYK GVSKSVIKTM SVMDFEKVNL ELIEALLEWI VDGKHAYPPG AVLVFLPGLA EIKMLYEQLQ SNSLFNNRRS HRCVIHPLHS SLSSEEQQAV FVKPPMGVTK IIISTNIAET SITIDDVVYV IDSGKMKEKR YDAGKGMESL EDTFVSQANA LQRKGRAGRV ASGVCFHLFT SHHYNHQLLK QQLPEIQRVP LEQLCLRIKI LEMFSTHNLQ SVFSRLIEPP HIDSLRASKV RLRDLGALTP DEKLTPLGYH LASLPVDVRI GKLMLLGSIF RCLDPALTIA ASLAFKSPFV SPWDKKEEAN QKKLEFAFAN SDYLALLCAY KGWQLSTKES ARASYNYCRQ NFLSGRTLQE MASLKRQFTE LLSDIGFVKE GLRAKEIEKR AQGGDGVLDA TGEEANTNAE NPKLISAVLC AALYPNVVQV KTPEGKFQKT SSGVVRLQPK SAELKFVTKN DGYVHIHPSS VNYQVRHFDS PYLLYHEKIK TSRVFIRDCS MVSVYPLVLF GGGQVNVQLQ RGAFVVSLDD GWIRFVAASH QVAELVKELR CELDQLLQDK IKNPSMDLCS CPRGSRIISM IVKLITTQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DHX57 (DEAH (Asp-Glu-Ala-Asp/His) Box Polypeptide 57 (DHX57))

Autre désignation: Dhx57 (DHX57 Produits)

Synonymes: fc68d07 Protein, wu:fc68d07 Protein, si:ch211-250c4.7 Protein, MGC186162 Protein, DDX57 Protein, AW494914 Protein, DExH-box helicase 57 Protein, DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 Protein, DEAH-box helicase 57 Protein, Dhx57-like DEXH-box helicase, probable Protein, Dhx57 Protein, DHX57 Protein, dhx57 Protein

Sujet: Putative ATP-dependent RNA helicase DHX57 (EC 3.6.4.13) (DEAH box protein 57),FUNCTION: Probable ATP-binding RNA helicase.

Poids moléculaire: 155.8 kDa

UniProt: Q6P5D3