PLEKHH2 Protein (AA 1-1491) (Strep Tag)

N° du produit ABIN3136350

Détail du produit

Antigène: PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1491

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PLEKHH2 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MEEPSEPEGL IDWKERCVAL EAQLMKFRVQ ASKIRELLAD KMQQLERQVI DAERQAEKAF QEVQVMEEKL KAANIQTSES ETRLYKKCQD LESVMQEKDD IIQNLALRLE EQKQVRIQEA KIIEEKAAKI KEWVTVKLNE LEVENQNLRF INQTQTEEIR AIQSKLQELQ EKKISCVSSP KTSEGQRNLT FGCFLSRAKS PPCVVRCEEV SKMASNEPEI TEGRCVEEME IAEKPADNQV QENSRSQRKL HETSCSSEQN QKTRASFAMD GGTSQNSGVP VSDWSSDEDD GSKGRSKSRC TSTLSSHTSE EGGQCGRLGS EAYLTASDDS SSIFEEETFD GNRPEQKKLC SWQQKAPWKA QGNLAKGRSQ SGVKEQDSSS DELNKKFHSQ RLDYTSSSSE ANTPSPILTP ALTPRYPNSL PGKGGAPLVP PPFQPPPKLR VPNVFSISVA LTKRHLSQPQ LCSDRMFGTN RNAISMIRPL RPQETDLDVV DGDGAEAVNR MDTGCDDGLF SYDSQDPPPC ADDQENSEAP KKAPCNKPPT PPLHRFPSWE SRIYAVAKSG IRVSEAFNME HANKNSADIL SYSAASLYTS LIYKNMTTPV YTTLKGKATQ ISSSPFLDDS SGSDEEDSSR SSSRLSESDA RSRSGPSSPR AMKRGVSDSS AASESDYAIP PDAYPIDAEC SQPEQKLLKT CLASCDNGKN EPLEKSGYLL KMSVRVKTWK RRWFVLKGGE LLYYKSPSDV IRKPQGHIEL SASCSILRGD NKQTVQLATE KHTYYLTADS PNILEEWIKV LQSVLRVQAA NPLCLQPEGK PAVKGLLTKV KHGYSKRVWC MLVGKVLYYF RNQEDKFPLG QLKLWEAKVE EVDRSCDSDE DYETRGCYLL STHYTIIVHP KDQGPTYLLI GSKHEKEAWL YHLTVAAGSN NINVGSEFEQ LVCKLLNIEG EPSSQIWRHP MLCHSKEGIL SPLTTLPSEA LQTEAIKLFK TCQLFINAAV DSPAIDYHIS LAQSALQVCL THPELQNEIC CQLIKQTRRR QLQNQPGPLQ GWQLLALCVG LFLPHHPFLW LLQLHLQRNA DSRTEFGKYA IYCQRCVERT QQNGDREARP SRMEILSTLL RNPYHHSRPF SIPVHFMNGL YQVVGFDAST TVEEFLNTLN QDTGMRKPAQ SGFALFTDDP SGRDLEHCLQ GNIKICDIIS KWEQASKEQQ PGKCEGSRTV RLTYKNRLYF SVQARGETDR EKTLLLYQTN DQIINGLFPL NKDLALEMAA LLAQVDIGDF ERPFSTPAGP VTNQCKANQT LKQVIERFYP KRYREGCSEE QLRQLYQRLS TKWMALRGHS AADCIRIYLT VARKWPFFGA KLFFAKPIAP SSLGNNCVWL AVHENGLSIL EYTSMRLVTS YMYKGLMTFG GYQEDFMVVV STQSKDRPTE KLLFAMAKHK ILEITLLIAS YINSFHQQKT TFHHLSAPAL LSPRTQAPQA RVMGSQPPLS NSRPTKGPTL L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))

Autre désignation: Plekhh2 (PLEKHH2 Produits)

Synonymes: RGD1304935 Protein, si:ch73-105b23.3 Protein, PLEKHH1L Protein, AI256725 Protein, E030001K05 Protein, mKIAA2028 Protein, pleckstrin homology, MyTH4 and FERM domain containing H2 Protein, pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 Protein, Plekhh2 Protein, plekhh2 Protein, PLEKHH2 Protein

Sujet: Pleckstrin homology domain-containing family H member 2,FUNCTION: In the kidney glomerulus may play a role in linking podocyte foot processes to the glomerular basement membrane. May be involved in stabilization of F-actin by attenuating its depolymerization. Can recruit TGFB1I1 from focal adhesions to podocyte lamellipodia. {ECO:0000269|PubMed:22832517}.

Poids moléculaire: 167.7 kDa

UniProt: Q8C115