PASK Protein (AA 1-1383) (Strep Tag)

N° du produit ABIN3136427

Détail du produit

Antigène: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1383

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PASK protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEDRGPPVFA EDWKCLSESP PVQEGPAAQA TFEPSKPLSI AHKHLSRKNG LSRLCQSRMA LSEDRWSSYC LSSLAAQNIC TSKLHCAAAP EYADPTAGPL GSTSCCSLLR GLASGCSGSL LSTPVCNPNK AVFTVDAKTT EILVANDKAC SLLGYSSHDL IGQKLAQFFL KSDSEVVEAL SEEHVEADGH AAVVFGTVVD IVSRIGEKIP VSVWIKRLQQ DRGLCCVVVL EPVERVSAWV AFQSDGTITS CDSLFAHLHG FTSPKDVVGQ CVIDLIPSMQ LPPPGQHIPK SLKIQRSVGR ARDGTTFPLS LKLKSKPSGR AVADSEAASE PGYQASVWVF CTISGLITLL PDGTIYGVNH SFALMLFGYG KTELLGKNIT FLIPGFYHYM DLTYDSSVQL PDLVNCLDIG RKSGPGEMNS DAQHNWELAS GAQGPRIDVV LARDHMPSQD ETLKLVGGQV SSRTQTRLET GYKILPSSAC QPSLGVDSNP EDGEQSLLTD QQSIPKRNLP AHGGQNQLDT SEISLPVLKE HLLSEIQKNI SEESPLTHRK WLSKVQQNPT KGSLPIHEEQ LLFAGQHIHV LGKEDPSAAE SYRESLLEES KSKPVDAKLF ASCEDSEPLV SVKDRGSSVD TCNLHQEAQL ELMGVSSPNP WADATMPEPH TTGQIAGGSL TYCPQYRSEW ASQQRGQDSA PSPSGMACVL LGTPTLDEPW PGVRNDREEL QTCLIKEQLS KSSCEGNLGI SRVELVPEEH PPFTAPVSFC DLGGRDLHAS RSGSSSACYA LATDLPGVLE AVEAQEADVN SYSWNLKELF LKDQTDRTPS HCSCTTSELS EAPSLSVVGS DLDVGILHRQ TSDILVDREM LLLTGTYFDL SEGQRFQEMG AGHDRAELSN ISLVSSEHYE TSDIESPGCD PPLPDPGPND MCLSAEKPRP SAQITSTPVA RGATSLQQEI QEGIYSGSCY HRDGLQLSIQ FEVKRVELQG SATLFCCWLV KDLFHSHRDS ATRTRLFLAS LPSSTHSMPE LSGSSFGEVL RAKPWFEESP TPAELEGLAA CEGEYDYKYN TISPLGSGAF GFVWTAVEKE CNKEVVVKFI KKEKVLEDCW IEDPKLGRVT LEIAILSKVD HANIIKVLDI FENQEFFQLV MEKHGSGMDL FAFIDHHPCL DEPLASFIFR QLVSAVGYLH SQGIIHRDIK DENIVIAEDF TIKLIDFGSA AYLERGKLFY TFCGTIEYCA PEVLIGNPYR GPELEMWSLG VTLYTLIFEE NPFCEVEETM EAVIHPPFLV SQELMSLLSG LLQPCPEQRT TLEKLIRDPW VTQPVNLASY TWEEVCRTNQ PESGLLSAAS LEIGSRSPSE MAQREGLCGP PAPRETRGDQ HCLHLKDPSL PVS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Autre désignation: Pask (PASK Produits)

Synonymes: pas Protein, wu:fb69h07 Protein, PASK Protein, PASKIN Protein, STK37 Protein, mKIAA0135 Protein, PAS domain containing serine/threonine kinase Protein, Pask Protein, pask Protein, PASK Protein

Sujet: PAS domain-containing serine/threonine-protein kinase (PAS-kinase) (PASKIN) (EC 2.7.11.1),FUNCTION: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells, however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation. {ECO:0000269|PubMed:15148392, ECO:0000269|PubMed:17878307, ECO:0000269|PubMed:18509100, ECO:0000269|PubMed:21181396}.

Poids moléculaire: 151.3 kDa

UniProt: Q8CEE6

Pathways: Regulation of Carbohydrate Metabolic Process