RAPGEF2 Protein (AA 1-1496) (Strep Tag)

N° du produit ABIN3136471

Détail du produit

Antigène: RAPGEF2 (Rap Guanine Nucleotide Exchange Factor (GEF) 2 (RAPGEF2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1496

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette RAPGEF2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MKPLAAPANH GVLGQQEKQS LPADFTKLHL TDSLHPQVTH VSSSHSGCSI TSDSGSSSLS DIYQATESEA GDMDLSGLPE TAVDSEDDDD EEDIERASDP LMSRDIVRDC LEKDPIDRTD DDIEQLLEFM HQLPAFANMT MSVRRELCAV MVFAVVERAG TIVLNDGEEL DSWSVILNGS VEVTYPDGKA EILCMGNSFG VSPTMDKEYM KGVMRTKVDD CQFVCIAQQD YCRILNQVEK NMQKVEEEGE IVMVKEHREL DRTGTRKGHI VIKGTSERLT MHLVEEHSVV DPTFIEDFLL TYRTFLSSPM EVGKKLLEWF NDPSLRDKVT RVVLLWVNNH FNDFEGDPAM TRFLEEFENN LEREKMGGHL RLLNIACAAK AKRRLMTLTK PSREAPLPFI LLGGSEKGFG IFVDSVDSCS KATEAGLKRG DQILEVNGQN FENIQLSKAM EILRNNTHLS ITVKTNLFVF KELLTRLSEE KRNGAPHLPK IGDIKKASRY SIPDLAVDVE QVIGLEKVNK KSKANTVGGR NKLKKILDKT RISILPQKPY NDIGIGQSQD DSIVGLRQTK HIPAALPVSG TLSSSNPDLL QSHHRILDFS TTPDLPDQVL RVFKADQQSR YIMISKDTTA KEVVIQAIRE FAVTATPEQY SLCEVSVTPE GVIKQRRLPD QLSKLADRIQ LSGRYYLKNN METETLCSDE DAQELLRESQ ISLLQLSTVE VATQLSMRNF ELFRNIEPTE YIDDLFKLKS KTSCANLKKF EEVINQETFW VASEILRETN QLKRMKIIKH FIKIALHCRE CKNFNSMFAI ISGLNLAPVA RLRTTWEKLP NKYEKLFQDL QDLFDPSRNM AKYRNVLSGQ NLQPPVIPLF PVIKKDLTFL HEGNDSKVDG LVNFEKLRMI AKEIRHVGRM ASVNMDPALM FRTRKKKWRS LGSLSQGSAN ATVLDVAQTG GHKKRVRRSS FLNAKKLYED AQMARKVKQY LSNLELEMDE ESLQTLSLQC EPATSTLPKN PGDKKPVKSE TSPVAPRAGP QQKVQPQQPL AQPQPPHKVS QGLQVPAVSL YPSRKKVPVK DLPPFGINSP QALKKILSLS EEGSLERHRK QAEDTISNAS SQLSSPPTSP QSSPRKGYAL ALSGTVDNFS DSGHSEISSR SSIVSNSSFD SVPVSLHDER RQRHSVSIVE SNLGVGRMER RTLMEPDQYS LGSYAPVSES RGLYAAATVI SSPSTEELSH DQGDRASLDA ADSGRGSWTS CSSGSHDNIQ TIQHQRSWET LPFGHTHFDY SGDAASIWAS GGHMDQMMFS DHSTKYNRQN QSRESLEQAQ SRASWASSTG YWGEDSEGDT GTIKRRGGKD VSAEAESSSM VPVTTEEAKP VPMPAHIAVT PSTTKGLIAR KEGRYREPPP TPPGYVGIPI ADFPEGPCHP ARKPPDYNVA LQRSRMVARP TEAPAPGQTP PAAAASRPGS KPQWHKPSDA DPRLAPFQPQ GFAGAEEDED EQVSAV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RAPGEF2 (Rap Guanine Nucleotide Exchange Factor (GEF) 2 (RAPGEF2))

Autre désignation: Rapgef2 (RAPGEF2 Produits)

Synonymes: MGC83978 Protein, RAPGEF2 Protein, fa11h07 Protein, wu:fa11h07 Protein, CNrasGEF Protein, NRAPGEP Protein, PDZ-GEF1 Protein, PDZGEF1 Protein, RA-GEF Protein, Rap-GEP Protein, nRap GEP Protein, 5830453M24Rik Protein, CNRasGEF Protein, Pdzgef1 Protein, RA-GEF-1 Protein, mKIAA0313 Protein, nRapGEP Protein, Rap guanine nucleotide exchange factor 2 Protein, Rap guanine nucleotide exchange factor 2 L homeolog Protein, Rap guanine nucleotide exchange factor (GEF) 2 Protein, RAPGEF2 Protein, rapgef2.L Protein, rapgef2 Protein, Rapgef2 Protein

Sujet: Rap guanine nucleotide exchange factor 2 (Cyclic nucleotide ras GEF) (CNrasGEF) (Neural RAP guanine nucleotide exchange protein) (nRap GEP) (PDZ domain-containing guanine nucleotide exchange factor 1) (PDZ-GEF1) (RA-GEF-1) (Ras/Rap1-associating GEF-1),FUNCTION: Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions. {ECO:0000269|PubMed:16272156, ECO:0000269|PubMed:17826737, ECO:0000269|PubMed:19453629, ECO:0000269|PubMed:19635461, ECO:0000269|PubMed:21864586, ECO:0000269|PubMed:23800469}.

Poids moléculaire: 166.4 kDa

UniProt: Q8CHG7

Pathways: Neurotrophin Signaling Pathway