MIB2 Protein (AA 1-973) (Strep Tag)

N° du produit ABIN3136681

Détail du produit

Antigène: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-973

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MIB2 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MDLDPHAGVQ VGMRVVRGMD WKWGQQDGGE GGVGTVVELG RHGSPSTPDR TVVVQWDQGT RTNYRAGYQG AHDLLLYDNA QIGIRHPNII CDCCKKHGLR GMRWKCRVCF DYDLCTQCYM HNKHDLTHAF ERYETSHSRP VTLSPRQGLP RIPLRGIFQG AKVVRGPDWE WGSQDGGEGK TGRVVDIRGW DVETGRSVAS VTWADGTTNV YRVGHKGKVD LRCVGEAAGG FYYKEHLPKL GKPAELQRRV SADGQPFQRG DKVKCLLDTD VLRDMQEGHG GWNPRMAEFI GQMGTVHRIT DRGDVRVQFN HETRWTFHPG ALTKHNSFWV GDVVRVIGDL DTVKRLQAGH GEWTDDMAPA LGRVGKVVKV FGDGNLRVAV GGQRWTFSPS CLVAYRPEED ANLDVAERAR ENKSAASVSV AGSLSVALDK LRTQKSDPEH PGRLVVEAAL GNVARALDLL RRHPEQASYH PALVVDTKNQ GRTALQVAAY LGQVELVRLL LQARASMDLP DDEGNTVLHY TAMGNQPEAT RVLLSAGCAV DARNGTRSTA LHVAVQRGFL EVVKILCERG CDVNLPDAHA DTPLHSAISA GAGASSIVEV LTEVPGIDVT ATNSQGFTLL HHASLKGHVL AVRKILARAR QLVDAKKEDG FTALHLAALN NHREVAQVLI REGRCDVNVR NRKLQSPLHL AVQQAHLGLV PLLVDAGCSV NTEDEEGDTA LHVALQRHQL LPLVADRAGG DPGPLQLLSR LQASGLPGCT ELTVGAAVAC FLALEGADVS YANHRGRSPL DLATEGRVLK ALQGCAQRFR ERQAGGGGGV PPGPRHVLST PNTVTNLHVS GTAGPEAAEC LVCSELALLI LFSPCQHRTV CEECARRMKK CIRCQVVISK KLRPDGSEVV NAIQVPGPPR QLVEELQSRY RQMEERITCP ICIDSHIRLV FQCGHGACAP CGAALNACPI CRQPIRDRIQ IFV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MIB2 (E3 ubiquitin-protein ligase MIB2 (MIB2))

Autre désignation: Mib2 (MIB2 Produits)

Synonymes: ZZANK1 Protein, ZZZ5 Protein, 2210008I11Rik Protein, Zzank1 Protein, skd Protein, mib2 Protein, mindbomb E3 ubiquitin protein ligase 2 Protein, mindbomb E3 ubiquitin protein ligase 2 S homeolog Protein, MIB2 Protein, Mib2 Protein, mib2 Protein, mib2.S Protein

Sujet: E3 ubiquitin-protein ligase MIB2 (EC 2.3.2.27) (Dystrophin-like protein) (Dyslike) (Mind bomb homolog 2) (Mind bomb-2) (RING-type E3 ubiquitin transferase MIB2) (Skeletrophin),FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. {ECO:0000269|PubMed:15824097}.

Poids moléculaire: 106.0 kDa

UniProt: Q8R516

Pathways: Signalisation Notch