PIK3R4 Protein (AA 2-1358) (His tag)

N° du produit ABIN3136713

Détail du produit

Antigène: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1358

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette PIK3R4 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: GNQLAGIAPS QILSVESYFS DIHDFEYDKS LGSTRFFKVA RAKHREGLVV VKVFAIQDPT LPLTSYKQEL EELKIRLHSA QNCLPFQKAA EKASEKAAML FRQYVRDNLY DRISTRPFLN NIEKRWIAFQ ILTAVDQAHK SGVRHGDIKT ENVMVTSWNW VLLTDFASFK PTYLPEDNPA DFNYFFDTSR RRTCYIAPER FVDGGMFATE LEYMRDPSTP LVDLNSNQRA RGELKRAMDI FSAGCVIAEL FTEGVPLFDL SQLLAYRNGH FFPEQVLNKI EDRSIRDLVT QMINREPEKR LEAEDYLKQQ RGNAFPEIFY TFLQPYMAQF AKETFLSADE RILVIRKDLG NIIHNLCGHD LPEKAEGESR ASGLVVLVSV ITSCLQTLKS CDSKLAALEL ILHLAPRLSV EILLDRITPY LLHFSNDSVP RVRAEALRTL TKVLALVQEV PRNDVNIYPE YILPGIAHLA QDDATIVRLA YAENIALLAE TALRFLELVQ LKTLNMENEP DNEEVDEATR PNGDYDTELQ ALHEMVQQKV VTLLSDPENI VKQTLMESGI TRLCVFFGRQ KANDVLLSHM ITFLNDKNDW HLRGAFFDSI VGVAAYVGWQ SSSILKPLLQ QGLSDAEEFV IVKALNALTC MCQLGLLQKP HVYEFASDIA PFLCHPNLWI RYGAVGFITV VAHQISTADV YCKLMPYLDP YITQPVIQIE RKLVLLSVLK EPVSRSIFDY ALRSKDIASL FRHLHMRQKK RNGSLLDCPP PEDPTIAQLL KKLLSQGMTE EEEDKLLALK DFMMKSNRAK ANAVDQSHLH DSSQKGVIDL AALGITGRQV DLVKTKQEPD EKRARKHVKQ DSNVNEEWKS MFGSLEPPNI PQALPKTSDH EVVQPGKPPR SESSAGICVP LSTSPQVSEA AHIPSKKPVI PVVSSTVLPS TYQIRITTCK TELQQLIQQK REQCNAERIA KQMMENAEWE SKPPPPGWRP KGLLVAHLHE HKSAVNRIRV SDEHLLFATC SNDGTVKIWN SQKMEGKTTT TRSILTYSRI GGRVKTLTFC QGSHYLAIAS DNGAVQLLGI EASKLPKSPK IHPLQSRILD QKEDGCVVDM HHFNSGAQSV LAYATVNGSL VGWDLRSSSN AWTLKHDLKS GLITSFAVDI HQCWLCIGTS SGAMACWDMR FQLPISSHCH PSRARIRRLS MHPLYQSWVI AAVQGNNEVS MWDMETGDRR LTLWASSAPP LSELQPSPHS VHGIYCSPAD GNPILLTAGS DMKIRFWDLV SPERSYVVAG STGSPSVSYY KKIIEGTEVV QEIQNKQKVG PSDDTPRRGP ESLPVGHHDI ITDIATFQTT QGFIVTASRD GIVKVWK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Pik3r4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Autre désignation: Pik3r4 (PIK3R4 Produits)

Synonymes: im:7148788 Protein, 2210010O15Rik Protein, C730038E05Rik Protein, C85833 Protein, D9Ertd418e Protein, VPS15 Protein, p150 Protein, phosphoinositide-3-kinase, regulatory subunit 4 Protein, phosphoinositide-3-kinase regulatory subunit 4 Protein, pik3r4 Protein, PIK3R4 Protein, Pik3r4 Protein

Sujet: Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate, different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (By similarity). {ECO:0000250|UniProtKB:Q99570}.

Poids moléculaire: 153.4 kDa Including tag.

UniProt: Q8VD65