ECE1 Protein (AA 1-769) (Strep Tag)

N° du produit ABIN3122356

Détail du produit

Antigène: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-769

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ECE1 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MRTVWSPLAA ALAALGMSTY KRATLDEEDL VDSLSEGDVY PNGLQVNFRS SRSGQRCWAA RTSVEKRLVV LVTLLAAGLV ACLAALGIQY QTRTPPVCLT EACVSVTSSI LNSMDPTVDP CQDFFSYACG GWIKANPVPD GHSRWGTFSN LWEHNQAVIK HLLENATASV SEAERKAQVY YRACMNETRI EELRAKPLME LIEKLGGWNI TGPWAKDNFQ DTLQVVTAHY RTSPFFSVYV SADSKNSNSN VIQVDQSGLG LPSRDYYLNK TENEKVLTGY LNYMVQLGKL LGGGDEDAIR PQMQQILDFE TALANITIPQ EKRRDEELIY HKVTAAELQT LAPAINWLPF LNTIFYPVEI NESEPIVVYD KEYLRQVSTL INNTDKCLLN NYMMWNLVRK TSSFLDQRFQ DADEKFMEVM YGTKKTCIPR WKFCVSDTEN NLGFALGPMF VKATFAEDSK NIASEIIMEI KKAFEESLST LKWMDEETRR SAKEKADAIY NMIGYPNFIM DPKELDKVFN DYTAVPDLYF ENAMRFFNFS WRVTADQLRK APNRDQWSMT PPMVNAYYSP TKNEIVFPAG ILQAPFYTRS SPNALNFGGI GVVVGHELTH AFDDQGREYD KDGNLRPWWK NSSVEAFKQQ TECMVQQYSN YSVNGEPVNG RHTLGENIAD NGGLKAAYRA YQNWVKKNGA EQTLPTLGLT SNQLFFLGFA QVWCSVRTPE SSHEGLITDP HSPSRFRVIG SLSNSKEFSE HFRCPPGSPM NPHHKCEVW
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Autre désignation: Ece1 (ECE1 Produits)

Synonymes: ECE Protein, Ece Protein, AW322500 Protein, ECE-1 Protein, ECE-1a Protein, ECE-1b Protein, ECE-1d Protein, ece1 Protein, MGC52874 Protein, MGC130791 Protein, fd11b07 Protein, wu:fd11b07 Protein, zgc:154079 Protein, endothelin converting enzyme 1 Protein, endothelin converting enzyme like 1 Protein, endothelin converting enzyme 1 L homeolog Protein, endothelin-converting enzyme 1 Protein, Endothelin-converting enzyme 1 Protein, ECE1 Protein, ECEL1 Protein, Ece1 Protein, ece1.L Protein, Za10_0551 Protein, Arch_0474 Protein, Closa_0961 Protein, Odosp_0578 Protein, Bacsa_1787 Protein, Weevi_2017 Protein, Fluta_3444 Protein, Sphch_2390 Protein, ece1 Protein

Sujet: Endothelin-converting enzyme 1 (ECE-1) (EC 3.4.24.71),FUNCTION: Converts big endothelin-1 to endothelin-1. {ECO:0000250}.

Poids moléculaire: 87.1 kDa

UniProt: Q4PZA2

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling