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CNR2 Protein (AA 1-347) (Strep Tag)

CNR2 Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
N° du produit ABIN3134162
  • Antigène Voir toutes CNR2 Protéines
    CNR2 (Cannabinoid Receptor 2 (CNR2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-347
    Origine
    • 6
    • 2
    • 1
    Souris
    Source
    • 3
    • 2
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification/Conjugué
    Cette CNR2 protéine est marqué à la Strep Tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA
    Séquence
    MEGCRETEVT NGSNGGLEFN PMKEYMILSS GQQIAVAVLC TLMGLLSALE NMAVLYIILS SRRLRRKPSY LFISSLAGAD FLASVIFACN FVIFHVFHGV DSNAIFLLKI GSVTMTFTAS VGSLLLTAVD RYLCLCYPPT YKALVTRGRA LVALCVMWVL SALISYLPLM GWTCCPSPCS ELFPLIPNDY LLGWLLFIAI LFSGIIYTYG YVLWKAHRHV ATLAEHQDRQ VPGIARMRLD VRLAKTLGLV LAVLLICWFP ALALMGHSLV TTLSDQVKEA FAFCSMLCLV NSMVNPIIYA LRSGEIRSAA QHCLIGWKKY LQGLGPEGKE EGPRSSVTET EADVKTT
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Attributs du produit
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    niveau d'endotoxine
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Top Product
    Discover our top product CNR2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CNR2 (Cannabinoid Receptor 2 (CNR2))
    Autre désignation
    Cnr2 (CNR2 Produits)
    Synonymes
    CNR2 Protein, CB2 Protein, cb2a Protein, cb2b Protein, CB-2 Protein, CX5 Protein, CB2C Protein, CNR2C Protein, CB2-R Protein, cannabinoid receptor 2 Protein, cannabinoid receptor 2 (macrophage) Protein, CNR2 Protein, cnr2 Protein, Cnr2 Protein
    Sujet
    Cannabinoid receptor 2 (CB-2) (CB2) (mCB2),FUNCTION: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. {ECO:0000269|PubMed:10822068, ECO:0000269|PubMed:11929767, ECO:0000269|PubMed:16407142, ECO:0000269|PubMed:16563625, ECO:0000269|PubMed:16924491, ECO:0000269|PubMed:17401376, ECO:0000269|PubMed:18286196, ECO:0000269|PubMed:8679694}.
    Poids moléculaire
    38.2 kDa
    UniProt
    P47936
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