ADCY8 Protein (AA 1-1249) (Strep Tag)

N° du produit ABIN3134718

Détail du produit

Antigène: ADCY8 (Adenylate Cyclase 8 (Brain) (ADCY8))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1249

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ADCY8 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MELSDVHCLS GSEELYTIQP TPPAGDDGSG SRPQRLLWQT AVRHITEQRF IHGHRGGGGG GVSRKASNPA GSGPNHHAPQ LSSDSVLPLY SLGPGERAHN TGGTKVFPER SGSGSASGSG GGGDLGFLHL DCAPSNSDFF LNGGYSYRGV IFPTLRNSFK SRDLERLYQR YFLGQRRKSE VVMNVLDVLT KLTLLVLHLS LASAPMDPLK GILLGFFTGI EVVICALVVV RKDNTSHTYL QYSGVVTWVA MTTQILAAGL GYGLLGDGIG YVLFTLFATY SMLPLPLTWA ILAGLGTSLL QVTLQVLIPR LAVFSINQVL AQVVLFMCMN TAGIFISYLS DRAQRQAFLE TRRCVEARLR LETENQRQER LVLSVLPRFV VLEMINDMTN VEDEHLQHQF HRIYIHRYEN VSILFADVKG FTNLSTTLSA QELVRMLNEL FARFDRLAHE HHCLRIKILG DCYYCVSGLP EPRRDHAHCC VEMGLSMIKT IRFVRSRTKH DVDMRIGIHS GSVLCGVLGL RKWQFDVWSW DVDIANKLES GGIPGRIHIS KATLDCLNGD YNVEEGHGKE RNEFLRKHNI ETYLIKQPEE SLLCLPEDIV KESVSCSDRR NSGATFTEGS WSPELPFDNI VGKQNTLAAL TRNSINLLPN HLAQALHVQS GPEEINKRIE HTIDLRSGDK LRREHIKPFS LMFKDSSLEH KYSQMRDEVF KSNLVCAFIV LLFITAIQSL LPSSRLMPMT IQFSILIMLH SALVLITTAE DYKCLPLILR KTCCWINETY LARNVIIFAS ILINFLGAVL NILWCDFDKS IPLKNLTFNS SAVFTDICSY PEYFVFTGVL AMVTCAVFLR LNSVLKLAVL LIMIAIYALL TETIYAGLFL SYDNLNHSGE DFLGTKEASL LLMAMFLLAV FYHGQQLEYT ARLDFLWRVQ AKEEINEMKE LREHNENMLR NILPSHVARH FLEKDRDNEE LYSQSYDAVG VMFASIPGFA DFYSQTEMNN QGVECLRLLN EIIADFDELL GEDRFQDIEK IKTIGSTYMA VSGLSPEKQQ CEDKWGHLCA LADFSLALTE SIQEINKHSF NNFELRIGIS HGSVVAGVIG AKKPQYDIWG KTVNLASRMD STGVSGRIQV PEETYLILKD QGFAFDYRGE IYVKGISEQE GKIKTYFLLG RVQPNPFILP PRRLPGQYSL AAVVLGLVQS LNRQRQKQLL NENSNSGIIK SHYNRRTLLT PSGPEPGAQA EGTDKSDLP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ADCY8 (Adenylate Cyclase 8 (Brain) (ADCY8))

Autre désignation: Adcy8 (ADCY8 Produits)

Synonymes: MGC121231 Protein, si:ch211-220f13.4 Protein, AC8 Protein, AW060868 Protein, ADCY3 Protein, HBAC1 Protein, Ac8 Protein, adenylate cyclase 8 (brain) S homeolog Protein, adenylate cyclase 8 Protein, adenylate cyclase 8 (brain) Protein, adenylate cyclase type 8 Protein, adenylate cyclase 3 Protein, adcy8.S Protein, ADCY8 Protein, adcy8 Protein, LOC100461383 Protein, Adcy8 Protein, ADCY3 Protein

Sujet: Adenylate cyclase type 8 (EC 4.6.1.1) (ATP pyrophosphate-lyase 8) (Adenylate cyclase type VIII) (Adenylyl cyclase 8) (Ca(2+)/calmodulin-activated adenylyl cyclase),FUNCTION: Catalyzes the formation of cAMP in response to calcium entry leadings to cAMP signaling activation that affect processes suche as synaptic plasticity and insulin secretion (PubMed:10864938, PubMed:25403481, PubMed:10482244, PubMed:14585998, PubMed:18448650). Plays a role in many brain functions, such as learning, memory, drug addiction, and anxiety modulation through regulation of synaptic plasticity by modulating long-term memory and long-term potentiation (LTP) through CREB transcription factor activity modulation (PubMed:10482244, PubMed:14585998, PubMed:18448650, PubMed:10864938, PubMed:12441059, PubMed:20638449, PubMed:27234425, PubMed:18222416). Plays a central role in insulin secretion by controlling glucose homeostasis through glucagon-like peptide 1 and glucose signaling pathway and maintains insulin secretion through calcium-dependent PKA activation leading to vesicle pool replenishment (PubMed:25403481). Also, allows PTGER3 to induce potentiation of PTGER4-mediated PLA2 secretion by switching from a negative to a positive regulation, during the IL1B induced-dedifferentiation of smooth muscle cells (By similarity). {ECO:0000250|UniProtKB:P40146, ECO:0000269|PubMed:10482244, ECO:0000269|PubMed:10864938, ECO:0000269|PubMed:12441059, ECO:0000269|PubMed:14585998, ECO:0000269|PubMed:18222416, ECO:0000269|PubMed:18448650, ECO:0000269|PubMed:20638449, ECO:0000269|PubMed:25403481, ECO:0000269|PubMed:27234425}.

Poids moléculaire: 140.1 kDa

UniProt: P97490

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma