FZD6 Protein (AA 19-709) (rho-1D4 tag)

N° du produit ABIN3135351

Détail du produit

Antigène: FZD6 (Frizzled Family Receptor 6 (FZD6))

Type de proteíne: Recombinant

Attributs du protein: AA 19-709

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette FZD6 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: HSLFTCEPIT VPRCMKMTYN MTFFPNLMGH YDQGIAAVEM GHFLHLANLE CSPNIEMFLC QAFIPTCTEQ IHVVLPCRKL CEKIVSDCKK LMDTFGIRWP EELECNRLPH CDDTVPVTSH PHTELSGPQK KSDQVPRDIG FWCPKHLRTS GDQGYRFLGI EQCAPPCPNM YFKSDELDFA KSFIGIVSIF CLCATLFTFL TFLIDVRRFR YPERPIIYYS VCYSIVSLMY FVGFLLGNST ACNKADEKLE LGDTVVLGSK NKACSVVFMF LYFFTMAGTV WWVILTITWF LAAGRKWSCE AIEQKAVWFH AVAWGAPGFL TVMLLAMNKV EGDNISGVCF VGLYDLDASR YFVLLPLCLC VFVGLSLLLA GIISLNHVRQ VIQHDGRNQE KLKKFMIRIG VFSGLYLVPL VTLLGCYVYE LVNRITWEMT WFSDHCHQYR IPCPYQANPK ARPELALFMI KYLMTLIVGI SAVFWVGSKK TCTEWAGFFK RNRKRDPISE SRRVLQESCE FFLKHNSKVK HKKKHGAPGP HRLKVISKSM GTSTGATTNH GTSAMAIADH DYLGQETSTE VHTSPEASVK EGRADRANTP SAKDRDCGES AGPSSKLSGN RNGRESRAGG LKERSNGSEG APSEGRVSPK SSVPETGLID CSTSQAASSP EPTSLKGSTS LPVHSASRAR KEQGAGSHSD A
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Fzd6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FZD6 (Frizzled Family Receptor 6 (FZD6))

Autre désignation: Fzd6 (FZD6 Produits)

Synonymes: zgc:65879 Protein, zgc:77440 Protein, fz6 Protein, frz6 Protein, Xfrz6 Protein, frizzled6 Protein, frizzled-6 Protein, FZ-6 Protein, FZ6 Protein, HFZ6 Protein, NDNC10 Protein, Frizzled-6 Protein, Fz6 Protein, frizzled class receptor 6 Protein, frizzled class receptor 6 S homeolog Protein, fzd6 Protein, fzd6.S Protein, FZD6 Protein, Fzd6 Protein

Sujet: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (By similarity). Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear. {ECO:0000250, ECO:0000269|PubMed:16495441}.

Poids moléculaire: 78.2 kDa Including tag.

UniProt: Q61089

Pathways: Signalisation WNT, Tube Formation