MST1R Protein (AA 311-1378) (rho-1D4 tag)

N° du produit ABIN3135521

Détail du produit

Antigène: MST1R (Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R))

Type de proteíne: Recombinant

Attributs du protein: AA 311-1378

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette MST1R protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: GAPEGTQPYP VLQAAHSAPV DAKLAVELSI SEGQEVLFGV FVTVKDGGSG MGPNSVVCAF PIYHLNILIE EGVEYCCHSS NSSSLLSRGL DFFQTPSFCP NPPGGEASGP SSRCHYFPLM VHASFTRVDL FNGLLGSVKV TALHVTRLGN VTVAHMGTVD GRVLQVEIAR SLNYLLYVSN FSLGSSGQPV HRDVSRLGND LLFASGDQVF KVPIQGPGCR HFLTCWRCLR AQRFMGCGWC GDRCDRQKEC PGSWQQDHCP PEISEFYPHS GPLRGTTRLT LCGSNFYLRP DDVVPEGTHQ ITVGQSPCRL LPKDSSSPRP GSLKEFIQEL ECELEPLVTQ AVGTTNISLV ITNMPAGKHF RVEGISVQEG FSFVEPVLTS IKPDFGPRAG GTYLTLEGQS LSVGTSRAVL VNGTQCRLEQ VNEEQILCVT PPGAGTARVP LHLQIGGAEV PGSWTFHYKE DPIVLDISPK CGYSGSHIMI HGQHLTSAWH FTLSFHDGQS TVESRCAGQF VEQQQRRCRL PEYVVRNPQG WATGNLSVWG DGAAGFTLPG FRFLPPPSPL RAGLVELKPE EHSVKVEYVG LGAVADCVTV NMTVGGEVCQ HELRGDVVIC PLPPSLQLGK DGVPLQVCVD GGCHILSQVV RSSPGRASQR ILLIALLVLI LLVAVLAVAL IFNSRRRKKQ LGAHSLSPTT LSDINDTASG APNHEESSES RDGTSVPLLR TESIRLQDLD RMLLAEVKDV LIPHEQVVIH TDQVIGKGHF GVVYHGEYTD GAQNQTHCAI KSLSRITEVQ EVEAFLREGL LMRGLHHPNI LALIGIMLPP EGLPRVLLPY MRHGDLLHFI RSPQRNPTVK DLVSFGLQVA CGMEYLAEQK FVHRDLAARN CMLDESFTVK VADFGLARGV LDKEYYSVRQ HRHARLPVKW MALESLQTYR FTTKSDVWSF GVLLWELLTR GAPPYPHIDP FDLSHFLAQG RRLPQPEYCP DSLYHVMLRC WEADPAARPT FRALVLEVKQ VVASLLGDHY VQLTAAYVNV GPRAVDDGSV PPEQVQPSPQ HCRSTSKPRP LSEPPLPT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Mst1r Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MST1R (Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R))

Autre désignation: Mst1r (MST1R Produits)

Synonymes: CD136 Protein, CDw136 Protein, PTK8 Protein, RON Protein, Fv-2 Protein, Fv2 Protein, Ron Protein, STK Protein, Cdw136 Protein, Ptk8 Protein, Stk Protein, macrophage stimulating 1 receptor Protein, macrophage stimulating 1 receptor (c-met-related tyrosine kinase) Protein, MST1R Protein, Mst1r Protein

Sujet: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

Poids moléculaire: 118.2 kDa Including tag.

UniProt: Q62190

Pathways: Signalisation RTK