FNDC3B Protein (AA 1-1207) (Strep Tag)

N° du produit ABIN3135716

Détail du produit

Antigène: FNDC3B (Fibronectin Type III Domain Containing 3B (FNDC3B))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1207

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette FNDC3B protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MYVTMMMTDQ IPLELPPLLN GEVAMMPHLV NGEAAQQVIL VQVNPGETFT IRAEDGTLQC IQGPAEVPMM SPNGSIPPIH VPPGYISQVI EDSTGVRRVV VTPQSPECYP PSYPSAMSPT HHLPPYLTHH PHFIQNSHTA YYPPVTVPGD MPPQFFPQPH LPPTIYSEPE IIPLYGMSSY VTREDQYSKP PHKKLKDRQI DRQNRLNSPP STIYKNSCAT VYNGYGKGHS GGSSGGGGGG SGGGPGIKKT ERRARSSPKS SDSDLQEYEL EVKRVQDILS GIEKPQVSNI QARAVVLSWA PPVGLSCGPH GGLSFPYSYE VALSDKGRDG KYKIIYSGEE LECNLKDLRP ATDYHVRVYA VYNSVKGSCS EPVSFTTHSC APECPFPPKL AHRSKSSLTL QWKAPIDNGS KITSYLLEWD EGKRNSGFRQ CFFGSQKHCK LTKLCPAMGY TFRLAARNDI GTSGYSQEVV CYTLGNIPQM PLAPRLVRAG VTWITLQWSR PEGCSPEEVI TYTLDIQEDE NDSHFHPKYT GEDLTCTVKN LKRSTQYKFR LTASNMEGKS CPSEVLVCTT SPDRPGPPTR PLIKGPVTSH GFSVKWDAPK DNGGSEIPKY LLEITDGTPE AGQWEVAYSG SATEYVFTHL KPGTLYKLRA CCISTGGHSQ CSESLPVRTL SLAPGQCRPP RVLGRPKHKG VHLEWDVPAS ESGCEVSEYS VEMTEPENVA SEVYHGPELE CTVGNLLPGT VYRFRVRALN DGGYGPYSDV SEITTAAGPP GQCRAPRVSF TPDGCVLVGW ESPASPGADI SEYRLEWGED EQSLELVYHG PDTCFEMRDL LPAAQYCCRL QAFNPAGAGP YSELVHCQTP ASAPDPVSTL CVLEEEPPSA HPDSPSVCLV LNWEEPCNNG SEILAYNIDL GDSCITVGNT TTHVMKNLLP ETTYRIRIQA INEIGVGPFS QFIKAKTRPL PPSPPRLECA ASGPQSLKLK WGDSNSKTHA AGDMVYTLQL EDRNKRFISI YRGPSHTYKV QRLTEFTCYS FRIQAMSEAG EGPYSETYTF STTKSVPPTL KAPRVTQLEG NSCEIFWETV PPMRGDPVSY VLQVLVGRDS EYKQVYKGEE ATFQISGLQS NTDYRFRVCA CRRCVDTSQE LSGAFSPSAA FMLQQREVML TGDLGGMEEA KMKGMMPTDE QFAALIVLGF ATLSILFAFI LQYFLMK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FNDC3B (Fibronectin Type III Domain Containing 3B (FNDC3B))

Autre désignation: Fndc3b (FNDC3B Produits)

Synonymes: FAD104 Protein, PRO4979 Protein, YVTM2421 Protein, 1600019O04Rik Protein, AW550168 Protein, Fad104 Protein, mKIAA4164 Protein, RGD1311673 Protein, FNDC3B Protein, DKFZp469D136 Protein, fibronectin type III domain containing 3B Protein, FNDC3B Protein, Fndc3b Protein, fndc3b Protein

Sujet: Fibronectin type III domain-containing protein 3B (Factor for adipocyte differentiation 104) (HCV NS5A-binding protein 37),FUNCTION: May be positive regulator of adipogenesis.

Poids moléculaire: 132.8 kDa

UniProt: Q6NWW9

Pathways: Positive Regulation of fat Cell Differentiation