TRPV6 Protein (AA 1-767) (Strep Tag)

N° du produit ABIN3136798

Détail du produit

Antigène: TRPV6 (Transient Receptor Potential Cation Channel, Subfamily V, Member 6 (TRPV6))

Type de proteíne: Recombinant

Attributs du protein: AA 1-767

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TRPV6 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MGPLQREDRP ALGGANVAPG SSPVGVWHQP QPPKEPAFHP MGWSLPKEKG LILCLWNKFC RWFHRQESWA QSRDEQNLLQ QKRIWESPLL LAAKENDVQA LSKLLKFEGC EVHQRGAMGE TALHIAALYD NLEAAMVLME AAPELVFEPM TSELYEGQTA LHIAVINQNV NLVRALLARG ASVSARATGS VFHYRPHNLI YYGEHPLSFA ACVGSEEIVR LLIEHGADIR AQDSLGNTVL HILILQPNKT FACQMYNLLL SYDGGDHLKS LELVPNNQGL TPFKLAGVEG NIVMFQHLMQ KRKHIQWTYG PLTSTLYDLT EIDSSGDDQS LLELIVTTKK REARQILDQT PVKELVSLKW KRYGRPYFCV LGAIYVLYII CFTMCCVYRP LKPRITNRTN PRDNTLMQQK LLQEAYVTPK DDLRLVGELV SIVGAVIILL VEIPDIFRLG VTRFFGQTIL GGPFHVIIIT YAFMVLVTMV MRLTNVDGEV VPMSFALVLG WCNVMYFARG FQMLGPFTIM IQKMIFGDLM RFCWLMAVVI LGFASAFYII FQTEDPDELG HFYDYPMALF STFELFLTII DGPANYDVDL PFMYSVTYAA FAIIATLLML NLLIAMMGDT HWRVAHERDE LWRAQVVATT VMLERKLPRC LWPRSGICGR EYGLGDRWFL RVEDRQDLNR QRIRRYAQAF QQQDGLYSED LEKDSGEKLE TARPFGAYLS FPTPSVSRST SRSSTNWERL RQGALRKDLR GIINRGLEDG EGWEYQI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TRPV6 (Transient Receptor Potential Cation Channel, Subfamily V, Member 6 (TRPV6))

Autre désignation: Trpv6 (TRPV6 Produits)

Synonymes: ecac Protein, trpv5-6 Protein, TRPV6 Protein, trpv6 Protein, CaT Protein, ECaC Protein, ABP/ZF Protein, CAT1 Protein, CATL Protein, ECAC2 Protein, HSA277909 Protein, LP6728 Protein, ZFAB Protein, CAT Protein, CaT1 Protein, Cac Protein, Ecac2 Protein, Otrpc3 Protein, transient receptor potential cation channel, subfamily V, member 6 Protein, transient receptor potential cation channel subfamily V member 6 Protein, trpv6 Protein, TRPV6 Protein, Trpv6 Protein

Sujet: Transient receptor potential cation channel subfamily V member 6 (TrpV6) (Calcium transport protein 1) (CaT1) (Epithelial calcium channel 2) (ECaC2),FUNCTION: Calcium selective cation channel that mediates Ca(2+) uptake in various tissues, including the intestine (PubMed:12765696, PubMed:12601087, PubMed:12574114, PubMed:14736889, PubMed:15123711, PubMed:17129178). Important for normal Ca(2+) ion homeostasis in the body, including bone and skin (PubMed:17129178, PubMed:22878123). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation (PubMed:15123711). In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner (PubMed:12601087). Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (PubMed:12574114). {ECO:0000269|PubMed:12574114, ECO:0000269|PubMed:12601087, ECO:0000269|PubMed:12660155, ECO:0000269|PubMed:12765696, ECO:0000269|PubMed:14736889, ECO:0000269|PubMed:15123711, ECO:0000269|PubMed:17129178, ECO:0000269|PubMed:22878123}.

Poids moléculaire: 87.4 kDa

UniProt: Q91WD2

Pathways: TCR Signaling